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Protocols
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DNA Isolating From Low Melting-Temperature Agarose
(NWFSC)
Phenol-based Method for the Isolation of DNA Fragments from Low-Melting Temperature Agarose
http://micro.nwfsc.noaa.gov/protocols/lmt.html
Added: Tue May 14 2002, Hits: 1702, Reviews: 0 Write review Cached -
DNA Isolation from Agarose Gel Using NaI
(Corbett Lab)
http://www.biochem.emory.edu/labs/acorbe2/protocol...
Added: Tue May 14 2002, Hits: 1213, Reviews: 0 Write review Cached -
DNA Isolation from Agarose Gels with DEAE Paper
(Hancock Lab)
Detailed protocol with recipes
http://www.cmdr.ubc.ca/bobh/methods/ISOLATIONOFDNA...
Added: Tue May 14 2002, Hits: 794, Reviews: 0 Write review Cached -
DNA Purification from Gels
(John Roth, Division of Biological Sciences,University of California at Davis)
Simple freezing method for recovering DNA from gels.
http://rothlab.ucdavis.edu/protocols/P02.html
Added: Tue May 25 2004, Hits: 2580, Reviews: 0 Write review Cached -
Elution of DNA fragments from agarose
(Roe, OU)
http://www.genome.ou.edu/protocol_book/protocol_pa...
Added: Tue May 14 2002, Hits: 3019, Reviews: 0 Write review Cached -
Elution of DNA Fragments from Agarose
(The Minion Lab, College of Veterinary Medicine at Iowa State University)
A protocol developed by Dr. Roe. First, the band of interest is excised, frozen at -70°C, Then, extracted with TE-saturated phenol and ethanol precipitated.
http://mycoplasmas.vm.iastate.edu/lab_site/methods...
Added: Tue Nov 16 2004, Hits: 1575, Reviews: 0 Write review Cached -
Freeze-Squeeze DNA Fragment Purification from Agarose Gels
(The Donelson Lab, University of Iowa)
http://www.biochem.uiowa.edu/donelson/Database%20i...
Added: Tue Apr 26 2005, Hits: 633, Reviews: 0 Write review Cached -
Gene-Kleen Protocol
(Dr. Simon Dawson, Department of Biochemistry, University of Nottingham Medical School)
Purification of DNA from agarose gels is an essential method involved in the sub-cloning of DNA fragments. This method describes a variation of the method of Vogelstein and Gillespie, 1979 (Proc. Natl. Acad. Sci. USA. 76, (2) 615-619) involving the use of sodium iodide.
http://www.nottingham.ac.uk/~mbzspd/methods/Gene-K...
Added: Sat Apr 10 2004, Hits: 547, Reviews: 0 Write review Cached -
Isolation of DNA from Agarose Gels (Paper Slurry Method)
(Kitto Lab, The University of Texas at Austin)
This procedure isolates DNA from agarose gels by filtration through a filter-paper column. The column is made in a 500 µL tube from a slurry of filter paper in TE buffer.
http://research.cm.utexas.edu/bkitto/Kittolabpage/...
Added: Tue Oct 08 2002, Hits: 855, Reviews: 0 Write review Cached -
Isolation of Restriction Fragments from Agarose Gels by Collection onto DEAE Cellulose
(Donis Keller Lab)
Recovery of DNA from agarose gel
http://humgen.wustl.edu/hdk_lab_manual/rdm/rdm10.h...
Added: Tue May 14 2002, Hits: 465, Reviews: 0 Write review Cached