Related Categories:
Protocols
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Dephosphorylation of Vector Plasmid
(Gerard Lazo)
http://wheat.pw.usda.gov/~lazo/methods/lazo/dephos...
Added: Tue May 14 2002, Hits: 4873, Reviews: 0 Write review Cached -
Preparing Vector for Blunt-end Ligation
(The Donelson Lab, University of Iowa)
Blunt ending vector
http://www.biochem.uiowa.edu/donelson/Database%20i...
Added: Tue Apr 26 2005, Hits: 3073, Reviews: 0 Write review Cached
Trouble Shootings and FAQs
- Double digestion of a vector - how to verify the success since only 14 bp fragment is removed?
- Couldn't digest my vector with an insert - Could be the ClaI used is dam methylation sensitive?
- Is dephosphorylation required for vector preparation?
- How to digest a vector with two restriction sites too close sited?
- Should I dephosphorylate my vector?