Protocols
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24h Subcloning Protocol
(Mirmira Laboratory at the University of Virginia)
This protocol is designed for subcloning inserts (I) from one vector into another vector (V). The inserts can be anywhere from 30 bp to 8 kb (possibly higher)
http://faculty.virginia.edu/mirmira/resources_file...
Added: Sat Jun 05 2004, Hits: 6867, Reviews: 0 Write review Cached -
DNA Subcloning
(Donis Keller Lab)
The method is used to clone smaller portions of inserts (up to 15 kb in size) which previously have been cloned in YACs phage cosmids or other plasmids.
http://humgen.wustl.edu/hdk_lab_manual/plasmid/pls...
Added: Tue May 14 2002, Hits: 7026, Reviews: 0 Write review Cached -
Easy Subcloning
(Michael Koelle)
The following protocols minimize the number of manipulations required to prepare DNA fragments for ligations, thereby both saving time and increasing reliability.
http://info.med.yale.edu/mbb/koelle/protocols/prot...
Added: Tue May 14 2002, Hits: 8575, Reviews: 1 Read reviews Write review Cached -
Subclong Strategies
(Mullins Lab, UCSF)
Useful tips on subcloning.
http://mullinslab.ucsf.edu/protocols/html/Typical_...
Added: Tue Nov 16 2004, Hits: 5766, Reviews: 0 Write review Cached -
Subcloning Protocol
(Laboratory of Cellular and Molecular Regulation)
This protocol describes preparation of vector, insert, ligation and transformation.
http://intramural.nimh.nih.gov/lcmr/snge/Protocols...
Added: Sun Jun 05 2005, Hits: 5075, Reviews: 0 Write review Cached