Protocols

• MicroRNA and siRNA Cloning Protocol (Bartel Lab, MIT)
  Detailed procedures on oligo design, synthesis, purification and cloning.
  http://web.wi.mit.edu/bartel/pub/protocols/miRNAcl...
  Added: Sat May 24 2003, Hits: 3710, Reviews: 1 Read reviews  Write review  

Trouble Shootings and FAQs

• How to ligate double-stranded oligos into a vector
• How to clone annealed oligos into a vector
• Oligo design strategy for cloning a gene into an expression vector
• Cloning using double-stranded oligos - Tips on preparation of oligos and vector, prevention of concatamer formation