Protocols
- cDNA Library Screening (Karl Clark, University of Minnesota)
Protocols for cDNA library screening which allows detection of expressed genes for subsequent cloning and sequencing. It contains the following sections: 1. Titer the library to determine the concentration of infectious phage; 2. Plate and lift the phage particles. 3. Hybridize the immobilized cDNA with labeled probe. 4. Pick positive plaques. 5. Cycle through steps 2-4 until a single cDNA containing phage is isolated, generally three cycles.
http://www.cbs.umn.edu/~kclark/protocols/library.h...
Added: Tue May 14 2002, Hits: 3010, Reviews: 0 Write review Cached -
Immunoscreening of Lambda GT11 Bacteriophage Libraries with Antibody Probes
( Dr. Bart Frank, Arthritis and Immunology Program, Oklahoma Medical Research Foundation)
http://omrf.ouhsc.edu/~frank/GT11.html
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Benton Davis Blots
( Dr. Bart Frank, Arthritis and Immunology Program, Oklahoma Medical Research Foundation)
Screening λgt recombinant clones by hybridization to single plaques in situ
http://omrf.ouhsc.edu/~frank/BENTNDAV.html
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cDNA & Genomic DNA Library Screening
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
http://www.pmci.unimelb.edu.au/core_facilities/man...
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cDNA Library Screening
(Laurie Lab, University of Virginia Health Science Center)
Conventional protocol for screening phage cDNA library, in great detail.
http://www.people.virginia.edu/~gwl6s/protocols/cd...
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cDNA Library Screening
(The Donelson Lab, University of Iowa)
http://www.biochem.uiowa.edu/donelson/Database%20i...
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Colony Hybridization
(Vesicle Trafficking)
http://cmgm.stanford.edu/~vesicle/protocols/hybrid...
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Expression Library Screening
(Dr. Volker Briese, Universitäts-Frauenklinik Rostock)
Procedures include Growing up host bacteria / Expression Library Titration /Plating Phage/ Screening Plaques with AP-Fusion Protein / In vivo Excision
http://www-ufk.med.uni-rostock.de/main/hmueller/la...
Added: Sun Nov 03 2002, Hits: 1076, Reviews: 0 Write review Cached -
PCR based DNA library screening method
(Methods.info)
This is a very useful method for the one who wants to screen a phage library non-radioactive and do not need too many clones. Up to 5 clones can be obtained usually within one week. However a prerequisite of this method is some knowledge in ?phage handling and a specific sequence you are searching for.
http://www.methods.info/Methods/Library_screening/...
Added: Wed Sep 04 2002, Hits: 1857, Reviews: 0 Write review -
Screening a Lambda Bacteriophage Genomic Library with a DNA Probe
( Dr. Bart Frank, Arthritis and Immunology Program, Oklahoma Medical Research Foundation)
http://omrf.ouhsc.edu/~frank/LIBSCRN.html
Added: Thu Sep 01 2005, Hits: 527, Reviews: 0 Write review Cached