Protocols

• BAC End-Sequencing (Caltech)
  Prepare BAC DNA for sequencing
  http://www.tree.caltech.edu/protocols/BAC_end_sequ...
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• BAC Manual (Texas A&M BAC Center)
  Provide details in the theory and practice for the construction, characterization, and screening of BAC libraries.
  http://hbz.tamu.edu/cgi-bin/htmlassembly?manual
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• Construction of BAC Library (Caltech)
  Guideline for BAC library preparation, the followings are described in great detail
  Preparation of BAC vector DNA
  Preparation of source DNA
  Ligation and Electroporation
  Preparation of BAC DNA
  http://www.tree.caltech.edu/protocols/BAC_lib_cons...
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• Growth and Small-Scale Isolation of BAC Clones (Pat Heslop-Harrison, Molecular Cytogenetics, University of Leicester)
  Method for growing up of BAC clones, making single colony preps and small-scale isolation of BAC DNA from 10 ml cultures
  http://www.le.ac.uk/biology/phh4/bacclone.htm
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• Hybridization of High Density Arrayed BAC Nylon Filter Blots (Caltech)
  Procedures for isolation and purification of cDNA inserts using polymerase chain reaction, probe labeling, probe hybridization and more...
  http://www.tree.caltech.edu/protocols/hi_density_s...
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• Large Scale Plasmid, Cosmid, BAC, PAC, and Fosmid DNA Isolation by a Cleared Lysate Method Followed (Roe Lab)
  http://www.genome.ou.edu/DblAcetateProcV3.html
  Added: Tue May 14 2002, Hits: 2153, Reviews: 0  Write review   Cached