Protocols
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Micro-Scale Isolation and Purification of Gangliosides
(Glycoteck)
This solvent partition method permits the isolation of gangliosides from small samples and from samples in which the ganglioside concentration is low, especially relative to the concentration of potentially contaminating proteins and other large molecular weight species. The method consists of three steps: (1) preparation of a dry total lipid extract, (2) partition of the total lipid extract in di-isopropyl ether/1-butanol/aqueous NaCl, and (3) Sephadex G-50 gel exclusion chromatography. The final total ganglioside preparation is lyophilized and stored in the dry state.
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