Protocols
- T and B cell Isolation (Contributed by Nanci E Donacki)
Isolate T and B cell from peripheral blood.
Added: Tue May 14 2002, Reviews: 0 Write review -
Differentiation to M1/M2 macrophages GM-CSF/M-CSF
(methodmint.com)
GM-CSF/M-CSF differentiation of CD14+ cells (isolated from monocytes) to M1/M2 macrophage subsets.
http://research.abl.es/methods/12/
Added: Sun Nov 06 2011, Hits: 711, Reviews: 0 Write review -
Erythrophagocytosis
(Viral Immunity & Pathogenesis Group, the Université Catholique de Louvain)
The erythrophagocytosis assay is performed to compare the phagocytic rate with and without anti-eythrocyte antibody in either macrophages from control animals or virus-infected counterparts.
http://www.icp.ucl.ac.be/mexp/vipg_eph.html
Added: Fri Mar 26 2004, Hits: 1190, Reviews: 0 Write review Cached -
Lymphoblastoid Cell Lines from Frozen Whole Blood
(Donis-Keller lab)
Blood Samples can be stored frozen as a backup in case an LCL is needed at a later date.
http://hg.wustl.edu/hdk_lab_manual/hcc/hcc10.html
Added: Tue May 14 2002, Hits: 446, Reviews: 0 Write review Cached -
Lymphocyte Transformation
(Donis-Keller lab)
Lymphocytes are transformed to establish cell lines. Mononuclear cells (lymphocytes) from anticoagulated venous blood are isolated by layering onto histopaque. During centrifugation, erythrocytes and granulocytes are aggregated by ficoll and rapidly settle to the bottom of the tube; lymphocytes and other mononuclear cells remain at the plasma-histopaque interface. Erythrocyte contamination is neglible. Most extraneous platelets are removed by low speed centrifugation during the washing steps.
http://hg.wustl.edu/hdk_lab_manual/hcc/hcc2.html
Added: Tue May 14 2002, Hits: 718, Reviews: 0 Write review Cached -
Macrophage Depletion by Clodronate
(Viral Immunity & Pathogenesis Group, the Université Catholique de Louvain)
Clodronate (dichloromethylene diphosphonate) is used for treatment of osteolytic diseases and osteoporosis Its effect is due to inhibittion of the function of osteoclasts. This drug is also taken up by macrophages and rapidly causes apoptosis of these cells.
http://www.icp.ucl.ac.be/mexp/vipg_clo.html
Added: Fri Mar 26 2004, Hits: 1025, Reviews: 0 Write review Cached -
Macrophage Extraction
(Lusis Lab, UCLA)
http://www.genetics.ucla.edu/labs/lusis/macrophage...
Added: Sat Mar 21 2009, Hits: 4137, Reviews: 0 Write review Cached -
Maintaining Lymphoblastoid Cell Lines
(Donis-Keller lab)
To grow lymphoblastoid cells for permanent storage and for DNA extraction.
http://hg.wustl.edu/hdk_lab_manual/hcc/hcc9.html
Added: Tue May 14 2002, Hits: 377, Reviews: 0 Write review Cached -
Monocytes/Macrophages: Enrichment of PBMCs with Monocytes
(Methods.info)
This protocol is used to reduce the costs of the cell sorting with MACS reagents. The cell suspension obtained after this protocol contains 40-70% monocytes. This cell suspension is than used for positive or negative MACS separation.
http://www.methods.info/Methods/Cell_biology/Macro...
Added: Sat Dec 20 2003, Hits: 2818, Reviews: 0 Write review Cached -
Needle Assay for Chemotaxis
(Devreotes Lab)
http://www.hopkinsmedicine.org/cellbio/devreotes/n...
Added: Tue May 14 2002, Hits: 760, Reviews: 0 Write review Cached -
Peritoneal Macrophage Preparation
(Viral Immunity & Pathogenesis Group, the Université Catholique de Louvain)
This well-written yet simple protocol is to prepare peritoneal macrophage cell for subsequent thrombophagocytosis test.
http://www.icp.ucl.ac.be/mexp/vipg_mfpre.html
Added: Sun Mar 21 2004, Hits: 3192, Reviews: 0 Write review Cached -
Phagocytosis
(Devreotes Lab)
http://www.hopkinsmedicine.org/cellbio/devreotes/p...
Added: Tue May 14 2002, Hits: 2096, Reviews: 0 Write review Cached -
Preparation of Lymphoblastoid Cell Lines for Long-Term Storage
(Donis-Keller lab)
Cell are frozen in RPMI-1640 with 15% Fetal Bovine Serum + 10% DMSO. Cultures are frozen slowly using a Model 700 Controller freezing chamber...
http://hg.wustl.edu/hdk_lab_manual/hcc/hcc3.html
Added: Tue May 14 2002, Hits: 355, Reviews: 0 Write review Cached -
Preparation of Lymphocyte Cell Pellet for Storage
(Donis-Keller lab)
Following propagation to 1 X 108 cells, lymphoblastoid cells are conveniently stored at -80 degrees C to preserve the high molecular weight DNA in the cells until the DNA is purified. This procedure describes the steps required to harvest and freeze the cells for long term storage.
http://hg.wustl.edu/hdk_lab_manual/hcc/hcc5.html
Added: Tue May 14 2002, Hits: 447, Reviews: 0 Write review Cached -
Purification of human mononuclear cells and neutrophils
(Springer Lab, Harvard University)
cell isolation
http://www.cbrinstitute.org/labs/springer/protocol...
Added: Wed Aug 14 2002, Hits: 1807, Reviews: 0 Write review Cached