Protocols
- T and B cell Isolation (Contributed by Nanci E Donacki)
Isolate T and B cell from peripheral blood.
Added: Tue May 14 2002, Reviews: 0 Write review -
Erythrophagocytosis
(Viral Immunity & Pathogenesis Group, the Université Catholique de Louvain)
The erythrophagocytosis assay is performed to compare the phagocytic rate with and without anti-eythrocyte antibody in either macrophages from control animals or virus-infected counterparts.
http://www.icp.ucl.ac.be/mexp/vipg_eph.html
Added: Fri Mar 26 2004, Hits: 1526, Reviews: 0 Write review Cached - In-vitro Phagocytosis Assay of Macrophages (Protocol Online)
This protocol is very useful for researchers working in native immunology field, which is very simple and here described with brief introduction and principle
Added: Tue Aug 21 2007, Reviews: 0 Write review -
Lymphoblastoid Cell Lines from Frozen Whole Blood
(Donis-Keller lab)
Blood Samples can be stored frozen as a backup in case an LCL is needed at a later date.
http://humgen.wustl.edu/hdk_lab_manual/hcc/hcc10.h...
Added: Tue May 14 2002, Hits: 1030, Reviews: 0 Write review Cached -
Lymphocyte Transformation
(Donis-Keller lab)
Lymphocytes are transformed to establish cell lines. Mononuclear cells (lymphocytes) from anticoagulated venous blood are isolated by layering onto histopaque. During centrifugation, erythrocytes and granulocytes are aggregated by ficoll and rapidly settle to the bottom of the tube; lymphocytes and other mononuclear cells remain at the plasma-histopaque interface. Erythrocyte contamination is neglible. Most extraneous platelets are removed by low speed centrifugation during the washing steps.
http://humgen.wustl.edu/hdk_lab_manual/hcc/hcc2.ht...
Added: Tue May 14 2002, Hits: 1996, Reviews: 0 Write review Cached -
Macrophage Depletion by Clodronate
(Viral Immunity & Pathogenesis Group, the Université Catholique de Louvain)
Clodronate (dichloromethylene diphosphonate) is used for treatment of osteolytic diseases and osteoporosis Its effect is due to inhibittion of the function of osteoclasts. This drug is also taken up by macrophages and rapidly causes apoptosis of these cells.
http://www.icp.ucl.ac.be/mexp/vipg_clo.html
Added: Fri Mar 26 2004, Hits: 1408, Reviews: 0 Write review Cached -
Maintaining Lymphoblastoid Cell Lines
(Donis-Keller lab)
To grow lymphoblastoid cells for permanent storage and for DNA extraction.
http://humgen.wustl.edu/hdk_lab_manual/hcc/hcc9.ht...
Added: Tue May 14 2002, Hits: 926, Reviews: 0 Write review Cached -
Monocytes/Macrophages: Enrichment of PBMCs with Monocytes
(Methods.info)
This protocol is used to reduce the costs of the cell sorting with MACS reagents. The cell suspension obtained after this protocol contains 40-70% monocytes. This cell suspension is than used for positive or negative MACS separation.
http://www.methods.info/Methods/Cell_biology/Macro...
Added: Sat Dec 20 2003, Hits: 6189, Reviews: 0 Write review Cached -
Needle Assay for Chemotaxis
(Devreotes Lab)
http://www.hopkinsmedicine.org/cellbio/devreotes/n...
Added: Tue May 14 2002, Hits: 1034, Reviews: 0 Write review Cached -
Peritoneal Macrophage Preparation
(Viral Immunity & Pathogenesis Group, the Université Catholique de Louvain)
This well-written yet simple protocol is to prepare peritoneal macrophage cell for subsequent thrombophagocytosis test.
http://www.icp.ucl.ac.be/mexp/vipg_mfpre.html
Added: Sun Mar 21 2004, Hits: 4911, Reviews: 0 Write review Cached