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Protocols
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Staining for Total Receptor
(Von Zastrow lab)
This protocol will visualize both internalized receptors that were once on the plasma membrane and receptors in the biosynthetic pathway. This protocol was specifically written for visualizing agonist-induced internalization of receptors.
http://www.ucsf.edu/vonzast/permeab.html
Added: Tue May 14 2002, Hits: 768, Reviews: 0 Write review Cached -
Staining Nonfilamentous Actin with Gc-Globulin
(Yu-li Wang's Lab, University of Massachusetts Medical School )
Includes direct and indirect methods
http://ylwang.umassmed.edu/protocol/cfs/dbp.htm
Added: Fri Oct 17 2003, Hits: 293, Reviews: 0 Write review Cached -
Vital Staining of Chromosomes with Hoechst 33258
(Yu-li Wang's Lab, University of Massachusetts Medical School )
http://ylwang.umassmed.edu/protocol/cfs/hoechst.ht...
Added: Fri Oct 17 2003, Hits: 1396, Reviews: 0 Write review Cached -
Yeast Immunofluorescence (with Methanol)
(D. Amberg Lab, Upstate Medical University)
This is the protocol to use with the Botstein anti-actin rabbit antibodies
http://www.upstate.edu/biochem/amberg/protocols/Me...
Added: Tue May 14 2002, Hits: 2102, Reviews: 0 Write review Cached