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Protocols
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Immunostaining of Cells
(Lamond Lab, University of Dundee )
Detailed protocol in PDF file and Quicktime movies.
http://www.lamondlab.com/f5immunostainprotocol.htm
Added: Thu May 08 2003, Hits: 3247, Reviews: 0 Write review Cached -
Immunostaining of Cells Adherent to Coverslips
(Laurie Lab, University of Virginia Health Science Center)
http://www.people.virginia.edu/~gwl6s/protocols/ce...
Added: Tue May 14 2002, Hits: 2619, Reviews: 0 Write review Cached -
Immunostaining of Cells Adherent to Coverslips
(iProtocol)
http://iprotocol.mit.edu/protocol/293.htm
Added: Tue Jul 27 2004, Hits: 1141, Reviews: 0 Write review Cached -
Introduction to Immunocytochemistry
(House Ear Institute)
A brief overview of common available methods.
http://www.hei.org/research/aemi/iem.htm
Added: Tue May 14 2002, Hits: 3859, Reviews: 0 Write review Cached -
Preparation of Colloidal Gold Probes
(House Ear Institute)
See how easily, and inexpensively, these expensive probes can be prepared. Excellent information if large volumes of gold probes are required.
http://www.hei.org/research/aemi/gold.htm
Added: Tue May 14 2002, Hits: 644, Reviews: 0 Write review Cached -
Problems with Immunolabeling
(House Ear Institute)
When labeling cells or sections with antibodies or other affinity markers, the results are not easily interpreted. Look here for some help.
http://www.hei.org/research/aemi/iempr.htm
Added: Tue May 14 2002, Hits: 1706, Reviews: 0 Write review Cached -
Silver Enhancement of Colloidal Gold
(House Ear Institute)
How to precipitate silver onto small colloidal gold particles
so that the signal can be detected by light microscopy.
http://www.hei.org/research/aemi/sil.htm
Added: Tue May 14 2002, Hits: 260, Reviews: 0 Write review Cached -
Using Resin Sections for Immunofluorescence
(House Ear Institute)
A protocol for applying antibodies with fluorescent markers onto sections of resin-embedded material. The thin sections help increase resolution.
http://www.hei.org/research/aemi/ifse.htm
Added: Tue May 14 2002, Hits: 477, Reviews: 0 Write review Cached