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Protocols

• Immunocytochemistry (The Science Advisory Board)
  Procedures for cell plating, fixing, antibody incubation and mounting
  http://www.scienceboard.net/resources/protocols.as...
  Added: Mon Feb 23 2009, Hits: 6791, Reviews: 0  Write review   Cached
• Embedding in Resins for Immunocytochemistry (House Ear Institute)
  Describes the use of the specialized and not so specialized resins.
  http://www.hei.org/research/aemi/resin.htm
  Added: Tue May 14 2002, Hits: 751, Reviews: 0  Write review   Cached
• Freeze Substitution for Embedding in Resins (House Ear Institute)
  A simple, inexpensive way of embedding in resin using freeze substitution methods.
  http://www.hei.org/research/aemi/pmfs.htm
  Added: Tue May 14 2002, Hits: 378, Reviews: 0  Write review   Cached
• Immunocytocheimical Identidication of Proliferating Cells in Vascular Tissue (anti-BrdU); Diaminoben (Wilcox Lab)
  http://www.emory.edu/WILCOX/brduDAB.html
  Added: Tue May 14 2002, Hits: 700, Reviews: 0  Write review   Cached
• Immunocytochemical Identification of Proliferating Cells in Vascular Tissue (anti-BrdU); Vector Red (Wilcox Lab)
  http://www.emory.edu/WILCOX/brdured.html
  Added: Tue May 14 2002, Hits: 476, Reviews: 0  Write review   Cached
• Immunocytochemistry Protocols (Lamond Lab, University of Dundee)
  In this section of Lamond Lab's collection of protocols, you can find the following protocols:
  - Conjugating antibodies
  - In vivo transcription
  - Paraspeckle staining
  - Cell fix - PFA
  - Cell fix - MeOH
  - Immunostaining (full protocol)
  - Preparing DNA/protein for microinjection
  - BrUTP labeling
  
  http://www.lamondlab.com/LTprotImmCyt.htm
  Added: Thu May 08 2003, Hits: 1198, Reviews: 0  Write review   Cached
• Immunocytohistochemistry (Howell Lab)
  Procedures for cell plating, fixing, antibody incubation and mounting
  http://cancer.ucsd.edu/howelllab/Immunocyto.html
  Added: Tue May 14 2002, Hits: 3117, Reviews: 0  Write review   Cached
• Immunostaining Cells on Coverslips (Cooper Lab, Department of Cell Biology & Physiology Washington University in St Louis)
  http://www.cooperlab.wustl.edu/LabMethodsReagentsO...
  Added: Fri Mar 20 2009, Hits: 1940, Reviews: 0  Write review  
• Immunostaining of Cells (Lamond Lab, University of Dundee )
  Detailed protocol in PDF file and Quicktime movies.
  http://www.lamondlab.com/f5immunostainprotocol.htm
  Added: Thu May 08 2003, Hits: 1007, Reviews: 0  Write review   Cached
• Immunostaining of Cells Adherent to Coverslips (Laurie Lab, University of Virginia Health Science Center)
  http://people.virginia.edu/~gwl6s/home.html/Method...
  Added: Tue May 14 2002, Hits: 1920, Reviews: 0  Write review   Cached
• Introduction to Immunocytochemistry (House Ear Institute)
  A brief overview of common available methods.
  http://www.hei.org/research/aemi/iem.htm
  Added: Tue May 14 2002, Hits: 2317, Reviews: 0  Write review   Cached
• Preparation of Colloidal Gold Probes (House Ear Institute)
  See how easily, and inexpensively, these expensive probes can be prepared. Excellent information if large volumes of gold probes are required.
  http://www.hei.org/research/aemi/gold.htm
  Added: Tue May 14 2002, Hits: 423, Reviews: 0  Write review   Cached
• Problems with Immunolabeling (House Ear Institute)
  When labeling cells or sections with antibodies or other affinity markers, the results are not easily interpreted. Look here for some help.
  http://www.hei.org/research/aemi/iempr.htm
  Added: Tue May 14 2002, Hits: 804, Reviews: 0  Write review   Cached
• Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips (R&D Systems, Inc.)
  Detailed protocol for fluorecent ICC staining is accompanied by a brief printable schematic.
  http://www.rndsystems.com/ihc_detail_objectname_fl...
  Added: Tue Jun 01 2010, Hits: 474, Reviews: 0  Write review   Cached
• Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells (R&D Systems, Inc.)
  A detailed protocol for preparation and fluorescent ICC staining of non-adherent cells is accompanied by a printable schematic.
  http://www.rndsystems.com/ihc_detail_objectname_fl...
  Added: Tue Jun 01 2010, Hits: 283, Reviews: 0  Write review   Cached
• Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections (R&D Systems, Inc.)
  A detailed protocol for gelatin-coated slide preparation is accompanied by a printable schematic
  http://www.rndsystems.com/ihc_detail_objectname_ge...
  Added: Tue Jun 01 2010, Hits: 170, Reviews: 0  Write review   Cached
• Silver Enhancement of Colloidal Gold (House Ear Institute)
  How to precipitate silver onto small colloidal gold particles
  so that the signal can be detected by light microscopy.
  http://www.hei.org/research/aemi/sil.htm
  Added: Tue May 14 2002, Hits: 349, Reviews: 0  Write review   Cached
• Using Resin Sections for Immunofluorescence (House Ear Institute)
  A protocol for applying antibodies with fluorescent markers onto sections of resin-embedded material. The thin sections help increase resolution.
  http://www.hei.org/research/aemi/ifse.htm
  Added: Tue May 14 2002, Hits: 438, Reviews: 0  Write review   Cached