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Protocols
- Immunocytochemistry (The Science Advisory Board)
Procedures for cell plating, fixing, antibody incubation and mounting
http://www.scienceboard.net/resources/protocols.as...
Added: Mon Feb 23 2009, Hits: 9102, Reviews: 0 Write review Cached -
Embedding in Resins for Immunocytochemistry
(House Ear Institute)
Describes the use of the specialized and not so specialized resins.
http://www.hei.org/research/aemi/resin.htm
Added: Tue May 14 2002, Hits: 856, Reviews: 0 Write review Cached -
Freeze Substitution for Embedding in Resins
(House Ear Institute)
A simple, inexpensive way of embedding in resin using freeze substitution methods.
http://www.hei.org/research/aemi/pmfs.htm
Added: Tue May 14 2002, Hits: 458, Reviews: 0 Write review Cached -
Immunocytocheimical Identidication of Proliferating Cells in Vascular Tissue (anti-BrdU); Diaminoben
(Wilcox Lab)
http://www.emory.edu/WILCOX/brduDAB.html
Added: Tue May 14 2002, Hits: 1307, Reviews: 0 Write review Cached -
Immunocytochemical Identification of Proliferating Cells in Vascular Tissue (anti-BrdU); Vector Red
(Wilcox Lab)
http://www.emory.edu/WILCOX/brdured.html
Added: Tue May 14 2002, Hits: 995, Reviews: 0 Write review Cached -
Immunocytochemistry Protocols
(Lamond Lab, University of Dundee)
In this section of Lamond Lab's collection of protocols, you can find the following protocols:
- Conjugating antibodies
- In vivo transcription
- Paraspeckle staining
- Cell fix - PFA
- Cell fix - MeOH
- Immunostaining (full protocol)
- Preparing DNA/protein for microinjection
- BrUTP labeling
http://www.lamondlab.com/LTprotImmCyt.htm
Added: Thu May 08 2003, Hits: 1198, Reviews: 0 Write review Cached -
Immunocytohistochemistry
(Howell Lab)
Procedures for cell plating, fixing, antibody incubation and mounting
http://cancer.ucsd.edu/howelllab/Immunocyto.html
Added: Tue May 14 2002, Hits: 4168, Reviews: 0 Write review Cached -
Immunostaining Cells on Coverslips
(Cooper Lab, Department of Cell Biology & Physiology Washington University in St Louis)
http://www.cooperlab.wustl.edu/LabMethodsReagentsO...
Added: Fri Mar 20 2009, Hits: 2961, Reviews: 0 Write review -
Immunostaining of Cells
(Lamond Lab, University of Dundee )
Detailed protocol in PDF file and Quicktime movies.
http://www.lamondlab.com/f5immunostainprotocol.htm
Added: Thu May 08 2003, Hits: 1637, Reviews: 0 Write review Cached -
Immunostaining of Cells Adherent to Coverslips
(Laurie Lab, University of Virginia Health Science Center)
http://people.virginia.edu/~gwl6s/home.html/Method...
Added: Tue May 14 2002, Hits: 2734, Reviews: 0 Write review Cached -
Introduction to Immunocytochemistry
(House Ear Institute)
A brief overview of common available methods.
http://www.hei.org/research/aemi/iem.htm
Added: Tue May 14 2002, Hits: 2592, Reviews: 0 Write review Cached -
Preparation of Colloidal Gold Probes
(House Ear Institute)
See how easily, and inexpensively, these expensive probes can be prepared. Excellent information if large volumes of gold probes are required.
http://www.hei.org/research/aemi/gold.htm
Added: Tue May 14 2002, Hits: 608, Reviews: 0 Write review Cached -
Problems with Immunolabeling
(House Ear Institute)
When labeling cells or sections with antibodies or other affinity markers, the results are not easily interpreted. Look here for some help.
http://www.hei.org/research/aemi/iempr.htm
Added: Tue May 14 2002, Hits: 986, Reviews: 0 Write review Cached -
Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
(R&D Systems, Inc.)
Detailed protocol for fluorecent ICC staining is accompanied by a brief printable schematic.
http://www.rndsystems.com/ihc_detail_objectname_fl...
Added: Tue Jun 01 2010, Hits: 1211, Reviews: 0 Write review Cached -
Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
(R&D Systems, Inc.)
A detailed protocol for preparation and fluorescent ICC staining of non-adherent cells is accompanied by a printable schematic.
http://www.rndsystems.com/ihc_detail_objectname_fl...
Added: Tue Jun 01 2010, Hits: 922, Reviews: 0 Write review Cached -
Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
(R&D Systems, Inc.)
A detailed protocol for gelatin-coated slide preparation is accompanied by a printable schematic
http://www.rndsystems.com/ihc_detail_objectname_ge...
Added: Tue Jun 01 2010, Hits: 704, Reviews: 0 Write review Cached -
Silver Enhancement of Colloidal Gold
(House Ear Institute)
How to precipitate silver onto small colloidal gold particles
so that the signal can be detected by light microscopy.
http://www.hei.org/research/aemi/sil.htm
Added: Tue May 14 2002, Hits: 510, Reviews: 0 Write review Cached -
Using Resin Sections for Immunofluorescence
(House Ear Institute)
A protocol for applying antibodies with fluorescent markers onto sections of resin-embedded material. The thin sections help increase resolution.
http://www.hei.org/research/aemi/ifse.htm
Added: Tue May 14 2002, Hits: 611, Reviews: 0 Write review Cached