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ELISA Inhibition Assay
(Goldberg Lab)
http://wheat.pw.usda.gov/~lazo/methods/goldberg/el...
Added: Tue May 14 2002, Hits: 4821, Reviews: 0 Write review Cached -
ELISA Procedure for Measuring Serum Antibody Titer
(Komabiotech)
the antigen (peptide or protein) is bound to the polystyrene microtiter plate first. The antiserum containing the anti-peptide antibody is then added to the well and allowed to bind. Finally, a second antibody, specific for the first antibody and labeled for detection, is added to the well and allowed to bind. The second antibody usually has an enzyme conjugated to it. This enzyme catalyzes the formation of colored substance from a colorless substrate. This colored substance is then quantified and the amount of antibody present can be calculated.
http://www.komabiotech.com/technical/protocol/ELIS...
Added: Tue May 14 2002, Hits: 6741, Reviews: 1 Read reviews Write review Cached -
ELISA Protocols
(Millipore)
Including Sandwich ELISA Method and Competitive ELISA Method
http://www.millipore.com/immunodetection/id3/elisa...
Added: Wed Feb 13 2008, Hits: 3295, Reviews: 0 Write review Cached -
ELISA with Platelet
(Viral Immunity & Pathogenesis Group, the Université Catholique de Louvain)
This modification of qualitative ELISA (Enzyme-Linked Immunosorbent Assay) is used for either screening detection of anti-platelet antibodies or for detection of platelet-associated Ig (PAIg)
http://www.icp.ucl.ac.be/mexp/vipg_plelisa.html
Added: Wed Mar 24 2004, Hits: 2199, Reviews: 0 Write review Cached -
Experiment Data Sheet
(Contributed by Nanci Donacki)
Useful sheet for recording ELISA data
http://www.protocol-online.org/protocols/experimen...
Added: Tue May 14 2002, Hits: 3693, Reviews: 0 Write review Cached - Gangliosides ELISA protocol (Contributed by Pingsunjim)
This protocol can be used for detection of gangliosides.Spicific antibodies to gangliosides are added to cells and allowed to bind to the cell surface. The nonbound antibody is washed away and an enzyme conjugated second antibody is then detected by the addition of an enzyme-spicific substrate.
Added: Tue May 14 2002, Reviews: 0 Write review -
Indirect ELISA
(Kitto Lab, The University of Texas at Austin)
The indirect ELISA is used primarily to determine the strength and/or amount of antibody response in a sample, whether it is from the serum of an immunized animal or the cell supernatant from growing hybridoma clones.
http://www.cm.utexas.edu/kitto/Kittolabpage/Protoc...
Added: Tue Oct 08 2002, Hits: 4974, Reviews: 0 Write review Cached -
Indirect ELISA Protocol Using Primary and Secondary Antibodies
(Abcam)
http://www.abcam.com/ps/pdf/protocols/Indirect%20E...
Added: Wed Jan 18 2006, Hits: 1255, Reviews: 0 Write review - Live Cell ELISA Protocol ( Contributed by Nanci Donacki )
Added: Tue May 14 2002, Reviews: 0 Write review - Peptide Inhibition ELISA Protocol (Contributed by Nanci Donacki)
Added: Tue May 14 2002, Reviews: 0 Write review