Protocols
- Sandwich ELISA Protocol (Chemicon)
Sandwich ELISAis used to determine the antigen concentration in unknown samples. This protocol describes both the principals and prcedures of sandwich ELISA, it also lists troubleshootings.
http://www.chemicon.com/resource/ANT101/a2C.asp
Added: Mon Apr 19 2004, Hits: 27938, Reviews: 0 Write review Cached -
ELISA Protocols
(Millipore)
Including Sandwich ELISA Method and Competitive ELISA Method
http://www.millipore.com/immunodetection/id3/elisa...
Added: Wed Feb 13 2008, Hits: 2202, Reviews: 0 Write review Cached -
Sandwich ELISA
(Kitto Lab, The University of Texas at Austin)
The sandwich ELISA measures the amount of antigen between two layers of antibodies. The antigens to be measured must contain at least two antigenic sites, capable of binding to antibody, since at least two antibodies act in the sandwich. So sandwich assays are restricted to the quantitation of multivalent antigens such as proteins or polysaccharides. Sandwich ELISAs for quantitation of antigens are especially valuable when the concentration of antigens is low and/or they are contained in high concentrations of contaminating protein.
http://research.cm.utexas.edu/bkitto/Kittolabpage/...
Added: Tue Oct 08 2002, Hits: 17955, Reviews: 0 Write review Cached -
Sandwich ELISA For Detecting Secreted ICAM-1
(Springer Lab, Harvard University)
http://www.cbrinstitute.org/investigators/springer...
Added: Wed Aug 14 2002, Hits: 5005, Reviews: 0 Write review Cached -
Sandwich ELISA Protocol Using a Pair of Primary Antibodies
(Abcam)
http://www.abcam.com/ps/pdf/protocols/Sandwich%20E...
Added: Wed Jan 18 2006, Hits: 5649, Reviews: 0 Write review