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Protocols
- TransWell Chemotaxis (Bio.com)
This protocol describes a method for studying chemotaxis (migration towards a concentration gradient of chemoattractant) of leukocytes (neutrophils, monocytes and lymphocytes) or other migratory cells.
http://bioprotocol.bio.com/protocolstools/protocol...
Added: Fri Oct 17 2003, Hits: 404, Reviews: 0 Write review Cached -
Ammonium Sulfate Binding Assay for Cells
(Devreotes Lab, John Hopkins Medical Institutions)
http://www.hopkinsmedicine.org/cellbio/devreotes/a...
Added: Thu Oct 03 2002, Hits: 435, Reviews: 0 Write review Cached -
Needle Assay for Chemotaxis
(Devreotes Lab, John Hopkins Medical Institutions)
http://www.hopkinsmedicine.org/cellbio/devreotes/n...
Added: Thu Oct 03 2002, Hits: 474, Reviews: 0 Write review Cached -
CHAPS Insoluble Floating Fraction Preparation
(Devreotes Lab, John Hopkins Medical Institutions)
Two methods are described
http://www.hopkinsmedicine.org/cellbio/devreotes/c...
Added: Thu Oct 03 2002, Hits: 369, Reviews: 0 Write review Cached -
Chemotaxis Assay
(Springer Lab, Harvard University)
The purpose of a chemotaxis assay is to determine whether your protein or small molecule of interest has chemotactic activity on a specific cell type. Chemotaxis is the ability of a protein to direct the migration of a specific cell. This assay is based on the premise of creating a gradient of the chemotactic agent and allowing cells to migrate through a membrane towards the chemotactic agent. If the agent is not chemotactic for the cell, then the majority of the cells will remain on the membrane. If the agent is chemotactic, then the cells will migrate through the membrane and settle on the bottom of the well of the chemotaxis plate.
http://labs.idi.harvard.edu/springer/protocols/mel...
Added: Sun Oct 25 2009, Hits: 428, Reviews: 0 Write review Cached -
GTPgS- induced Inhibition of Binding
(Devreotes Lab, John Hopkins Medical Institutions)
http://www.hopkinsmedicine.org/cellbio/devreotes/g...
Added: Thu Oct 03 2002, Hits: 339, Reviews: 0 Write review Cached -
In Vivo Actin Polymerization
(Devreotes Lab, John Hopkins Medical Institutions)
http://www.hopkinsmedicine.org/cellbio/devreotes/i...
Added: Thu Oct 03 2002, Hits: 90, Reviews: 0 Write review Cached -
In Vivo Adenylyl Cyclase Assay
(Devreotes Lab, John Hopkins Medical Institutions)
http://www.hopkinsmedicine.org/cellbio/devreotes/n...
Added: Thu Oct 03 2002, Hits: 72, Reviews: 0 Write review Cached