Related Categories:
Protocols
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Antibody Conjugation
(Immunology Applications Core Facility, University of Chicago)
This protocol can be used to couple either FITC or Biotin to IgG.
http://iacf.bsd.uchicago.edu/Monoclonal_Antibody_F...
Added: Mon Apr 19 2004, Hits: 2728, Reviews: 0 Write review Cached -
Antibody labeling
(House Ear Institute)
Labeling protocols using affinity markers.
http://www.hei.org/research/aemi/label.htm
Added: Tue May 14 2002, Hits: 2829, Reviews: 0 Write review Cached - Biotinylation of Antibody (Contributed by Nanci Donacki)
Added: Tue May 14 2002, Reviews: 0 Write review -
Protein (Antibody) Fluorescent Labeling
(Mario Roederer)
Detailed protocol for conjugation of proteins (principally monoclonal antibodies) to fluorescent dyes. The conjugations fall into four basic protocols: Type 1 (used for FITC, Cy5, and the initial preparation of the Cy5 and Cy7 tandem dyes); Type 2 (used for Biotin and Cascade Blue); Type 3 (used for conjugation of PE, APC, TR-BSA, and their derivatives); and Type 4 (used for the initial preparation of non-immunoglobulin proteins like Annexin V).
http://www.drmr.com/abcon/
Added: Tue May 14 2002, Hits: 3006, Reviews: 0 Write review Cached -
Protein (Antibody) Fluorescent Labeling
(Mario Roederer)
Gives detailed protocol for conjugation of proteins to fluorescent dyes. The conjugations fall into four basic protocols: Type 1 (used for FITC, Cy5, and the initial preparation of the Cy5 and Cy7 tandem dyes); Type 2 (used for Biotin and Cascade Blue); Type 3 (used for conjugation of PE, APC, TR-BSA, and their derivatives); and Type 4 (used for the initial preparation of non-immunoglobulin proteins like Annexin V). Procedures for the conjugation of immunoglobulins are provided for the following molecules:
http://www.drmr.com/abcon/
Added: Tue May 14 2002, Hits: 2072, Reviews: 0 Write review Cached