(Immunology Applications Core Facility, University of Chicago)
This protocol can be used to couple either FITC or Biotin to IgG.
Added: Mon Apr 19 2004, Hits: 159, Reviews: 0
(House Ear Institute)
Labeling protocols using affinity markers.
Added: Tue May 14 2002, Hits: 1429, Reviews: 0
Antibody Labeling of Cultured Cells
Basic Protocol for antibody labeling of cultured cells.
Added: Thu Oct 14 2010, Hits: 455, Reviews: 0
- Biotinylation of Antibody (Contributed by Nanci Donacki)
Added: Tue May 14 2002, Reviews: 0
Protein (Antibody) Fluorescent Labeling
Detailed protocol for conjugation of proteins (principally monoclonal antibodies) to fluorescent dyes. The conjugations fall into four basic protocols: Type 1 (used for FITC, Cy5, and the initial preparation of the Cy5 and Cy7 tandem dyes); Type 2 (used for Biotin and Cascade Blue); Type 3 (used for conjugation of PE, APC, TR-BSA, and their derivatives); and Type 4 (used for the initial preparation of non-immunoglobulin proteins like Annexin V).
Added: Tue May 14 2002, Hits: 2038, Reviews: 0