Protocols
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Immunfluoresence on Chamber Slides
(Bowtell Lab)
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Immunofluorescencc Microscopy of tissue culture cells
(Microscopy and Electronic Imaging Lab)
These methods are written for direct staining of filamentous actin with bodipy FL-phallicidin and indirect immunofluorescence staining of microtubules with anti-tubulin antibodies. They easily can be modified for conventional immunofluorescence staining of any tissue cultured cell or direct staining of actin filaments
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Mounting medium for immunofluorescence microscopy
(House Ear Institute)
This mounting medium is easy to prepare, can be stored cold or frozen, can be used with anti-fade agents and will polymerize to give a dry, semi-permanent mount. If for some reason the sample is needed again, immesing in water will dissolve the medium again.
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Photomicrography
(Bowtell Lab)
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