Protocols
- Specimen Preparation for Electron Microscopy (Veterinary Pathology, Bristol University)
This group of procedures describe almost every aspect of preparing sections for electron microscopy such as tissue processing, fixation, embedding, sectioning.
http://www.bris.ac.uk/Depts/PathAndMicro/CPL/emtec...
Added: Sun May 23 2004, Hits: 7284, Reviews: 0 Write review Cached -
DNA Spreading and Shadowing
(Optical Analysis Facility, University of Alabama)
DNA sample preparation for electron microscopy
http://bama.ua.edu/~emlab/instructions/dna_spreadi...
Added: Thu Mar 29 2007, Hits: 210, Reviews: 0 Write review Cached -
Fixation and Embedding of Microtubules for Electron Microscopy
(Microscopy and Electronic Imaging Lab)
(This procedure can also be used for virtually any material that must be pelleted prior to fixation and thin sectioning)
http://www.ku.edu/~bcmic/MEIL/techniques/micro_tub...
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Fixation of Cells Cultured in Transwell Dishes
(Microscopy and Electronic Imaging Lab)
Transwell culture dishes are commonly used to culture cells so that the top and bottom of the cells can be exposed to different culture media conditions. They are commonly used with polarized epithelial cells and endothelial cells. The cells are cultured on membrane supports and processing for transmission electron microscopy is greatly facilitated if the membranes are left intact and under the tension placed by the wells in the dish. Most dishes are destroyed by solvents used in embedding but the following method, when used with polycarbonate Transwell plates obtained from Costar, works very well.
http://www.ku.edu/~bcmic/MEIL/techniques/transwell...
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Preparation Of Ciliated Protozoa For Scanning Electron Microscopy
(Microscopy and Electronic Imaging Lab)
This procedures is used to fix and stain cells for SEM and for TEM. Cells can be fixed using conventional glutaraldehyde-osmium fixation To preserve ciliary orientation, use the "instant fixation" protocol described here. With this method, the cortex and ciliary beat form is well preserved but the cytoplasm is poorly preserved and membrane breakage and blebbing on the cell surface is evident.
http://www.ku.edu/~bcmic/MEIL/techniques/SEMtetr.h...
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Preparation of Yease Cell for ImmunoEletron Microscopy
(Jon Mulholland)
http://genome-www.stanford.edu/group/botlab/protoc...
Added: Tue May 14 2002, Hits: 525, Reviews: 0 Write review -
Sample Fixation for Electron Microscopy
(Microscopy and Electronic Imaging Lab)
This method produce good fixation for most tissues.
http://www.ku.edu/~bcmic/MEIL/techniques/generic.h...
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Sample Preparation for SEM
(Optical Analysis Facility, University of Alabama)
Methods for preparing animal tissues, plant tissues and cells for scanning electron microscopy.
http://bama.ua.edu/~emlab/instructions/sem_fix.htm...
Added: Thu Mar 29 2007, Hits: 965, Reviews: 0 Write review Cached -
Sample Preparation for TEM
(Optical Analysis Facility, University of Alabama)
Schedule for different types of samples and method for microwave fixation
http://bama.ua.edu/~emlab/instructions/tem_fix.htm...
Added: Thu Mar 29 2007, Hits: 920, Reviews: 0 Write review Cached -
Specimen Preparation for Scanning Electron Microscopy
(Microscopy and Electronic Imaging Lab)
Methods for preparation of a variety of specimens.
http://www.ku.edu/~bcmic/MEIL/techniques/SEMprep.h...
Added: Tue May 14 2002, Hits: 2171, Reviews: 0 Write review Cached