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Protocols

• Live imaging with Drosophila tissue culture cells (Epigenome NoE)  new
  Live imaging provides an important complementation to the "snapshot" view obtained in fixed tissue by immunofluorescence. It allows following dynamic cellular processes as they unfold "in vivo", and often reveals a degree of complexity impossible to study in still images. Thanks to the increasing interest in this technique, significant technical improvements have been made in the recent years in microscopy leading to more sensitive and faster cameras, more efficient filter sets and a variety of different microscope systems to choose from. The temporal dimension of cellular processes in microscopy is still often neglected due to the difficulties in technical setup and the necessity to fabricate your own tools. Now the majority of the required equipment is commercially available from microscope manufacturers and related companies including environmental chambers, CO2-control and temperature control.
  http://www.epigenome-noe.net/researchtools/protoco...
  Added: Mon Aug 04 2008, Hits: 0, Reviews: 0  Write review  
• How to Perform Automated Counts of Fluorescently Stained Cells (Fero Lab, Fred Hutchinson Cancer Research Center)
  This protocol describes semi-automated cell counts using fluorescently labeled cells, a hemocytometer and ImageJ software.
  http://www.fhcrc.org/science/labs/fero/Protocols/c...
  Added: Thu Jul 10 2008, Hits: 30, Reviews: 0  Write review