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Protocols

• Microsatellite Isolation (Srel DNA Lab, University of Georgia)
  Step by step protocol for microsatellite isolation.
  http://www.uga.edu/srel/DNA_Lab/Msat_Easy_Isolatio...
  Added: Mon Sep 23 2002, Hits: 1344, Reviews: 0  Write review   Cached
• Denaturing Urea-Polyacrylamide Gel Electrophoresis (PAGE) Based Microsatellite Analysis
  This protocol will give very good information for researchers working on microsatelite markers with brief introduction and principle of method
  Added: Mon Feb 02 2009, Reviews: 0  Write review  
• Enrichment for Microsatellite Sequences in Onion (Dirk Fischer, Dpt. Experimental Taxonomy of the Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben ,Germany)
  Microsatellite markers, also referred to as STMS (SequenceTagged Microsatellite Sites) or STR (Short Tandem Repeats) are widely used as molecular markers for intraspecific genotyping, molecular mapping and breeding purposes. The method is an efficient,fast and relatively inexpensive way to obtain microsatellite markers without post-cloning selection methods. So far, the method has been successful in onion (Allium cepa L.), a plant with a large genome and for pathogenic fungi.
  http://barley.ipk-gatersleben.de/~dirkf/home.html
  Added: Tue Sep 24 2002, Hits: 626, Reviews: 1 Read reviews  Write review  
• Microsatellite markers: isolation with non-radioactive probes and amplification (Département des Sciences Animales de l'Institut National Agronomique Paris-grignon)
  provide geneticians and ecologists with a reliable method for the isolation of microsatellites and the determination of basic conditions necessary to routinely use them as molecular markers. The actual protocol is a zipped word file.
  http://www.inapg.inra.fr/dsa/microsat/microsat.htm...
  Added: Mon Sep 23 2002, Hits: 979, Reviews: 0  Write review  
• Non-Radioactive Isolation and Amplification Protocols for microsatellite markers (Rob Toonen, University of California, Davis)
  http://biogeek.ucdavis.edu/MsatProtocolV1.pdf
  Added: Mon Sep 23 2002, Hits: 963, Reviews: 0  Write review  
• Preparation of denaturing polyacrylamide gels for microsatellite analysis (Pat Heslop-Harrison and Trude Schwarzacher, Department of Biology, University of Leicester)
  http://www.le.ac.uk/bl/phh4/acryl.htm
  Added: Sun Nov 01 2009, Hits: 395, Reviews: 0  Write review   Cached
• Quantitative Microsatellite Analysis (QuMA) (Chen Lab, UCSF)
  http://bts.ucsf.edu/chen/Protocol/QuantitativeMicr...
  Added: Sun Oct 25 2009, Hits: 545, Reviews: 0  Write review