Protocols
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cDNA-AFLP Protocol
(Delaney Lab at the University of Vermont)
http://www.uvm.edu/~tpdelane/lab/protocols/cDNA-AF...
Added: Fri Feb 06 2009, Hits: 551, Reviews: 0 Write review Cached -
Differential Display
(Chun-Ming Liu)
The following procedures are described:
RNA extraction and qualification DNase treatment of RNA sample
Reverse transcription
PCR amplification
Separation on acrylamide gels
DNA extraction from bands of interest
Re-amplification by PCR
Separation on agarose gels
Excision of amplified products
http://www.plant.dlo.nl/projects/hybtech/Liu/DISPL...
Added: Tue May 14 2002, Hits: 1492, Reviews: 0 Write review Cached -
Differential Display
(Breeden Lab)
Detailed protocol for differential display
http://www.fhcrc.org/labs/breeden/Methods/differen...
Added: Tue May 14 2002, Hits: 1044, Reviews: 0 Write review Cached -
Differential Display
(Clontech)
Provides detailed guide to differential display technique
http://www.clontech.com/images/pt/dis_manuals/PT11...
Added: Tue May 14 2002, Hits: 1480, Reviews: 0 Write review -
Differential Display-Reverse Transcription-PCR
(Gerard Lazo)
http://wheat.pw.usda.gov/~lazo/methods/lazo/ddrtpc...
Added: Tue May 14 2002, Hits: 550, Reviews: 0 Write review Cached -
Serial Analysis of Gene Expression
(SAGE)
SAGE is a powerful tool that allows the analysis of overall gene expression patterns with digital analysis. Because SAGE does not require a preexisting clone, it can be used to identify and quantitate new gene as well as know genes.
http://www.sagenet.org/
Added: Tue May 14 2002, Hits: 693, Reviews: 0 Write review Cached -
Targeted Differential Display
(Molecular Profiling Initiative, NCI)
Protocol for differential gene expression analysis of cells obtained from microdissected tissue.
http://cgap-mf.nih.gov/Protocols/DNARNAProteomicAn...
Added: Tue May 14 2002, Hits: 132, Reviews: 0 Write review Cached -
What's Differential Display
(GenHunter)
Introduction to differential display technique
http://www.genhunter.com/products/differential/
Added: Tue May 14 2002, Hits: 941, Reviews: 0 Write review Cached