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Protocols
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Screening cDNA Clones by RNA In Situ Hybridization
(Goodman Laboratory)
The RNA in situ procedure described below is based on the protocol developed by Tautz and Pfeifle, but adapted to allow the screening of 96 RNA probes on whole-mount Drosophila embryos at the same time.
http://www.fruitfly.org/about/methods/RNAinsitu.ht...
Added: Tue May 14 2002, Hits: 411, Reviews: 0 Write review Cached -
Storage of Chromosome Preparations for FISH
(Thomas Ried's Lab, NCI)
The quality of stored chromosome preparations varies considerably from one case to another. In some cases, good preparations can be obtained from cells stored in fixative, while in other cases, the quality of the chromosome spreads obtained from cell pellets is poor. It is therefore recommended that specimens harvested for chromosome preparations which are to be used for FISH and SKY be stored both as cell pellets and as slide preparations.
http://www.riedlab.nci.nih.gov/publications/2415.3...
Added: Fri Oct 18 2002, Hits: 884, Reviews: 0 Write review -
Tissue Preparation for In Situ Hybridization
(Wilcox Lab)
http://www.emory.edu/WILCOX/tissueprep.html
Added: Tue May 14 2002, Hits: 1924, Reviews: 0 Write review Cached