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Protocols
- In situ cytogenetics (alpha-satellite probe) (Matt Lewis, Department of Pathology, University of Liverpool)
Alpha satellite sequences, whilst highly repetitive, are specific to each individual chromosome. These sequences flank the centromeres and can present a target measured in megabases. In this protocol a biotin or digoxigenin labelled DNA probe is detected using HRP-conjugated antibodies. The signal is visualised with diaminobenzidine (DAB). Normal, healthy nuclei show two spots. Aneusomic nuclei show 1, 3, 4 or more spots.
http://www.methodbook.net/probes/insitu.html
Added: Fri Sep 06 2002, Hits: 957, Reviews: 0 Write review Cached -
Micronucleus Assay in Blood Lymphocytes
(Nina T. Holland, Division of Environmental Health Sciences, Berkeley)
Detailed procedures for lymphocyte isolation from blood, lymphocyte culture and micronucleus assay.
http://ehs.sph.berkeley.edu/holland/protocolLibrar...
Added: Wed Oct 02 2002, Hits: 2575, Reviews: 1 Read reviews Write review Cached -
Silver Staining for Nucleolar Organizer Regions
(Pat Heslop-Harrison, Molecular Cytogenetics, University of Leicester)
http://www.le.ac.uk/biology/phh4/agnor.htm
Added: Fri May 13 2005, Hits: 635, Reviews: 0 Write review Cached