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cDNA/AFLP
(CWB BACHEM)
A method for visualisation of differential gene expression
http://www.dpw.wau.nl/pv/staff/aflp.htm
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Clonality: X-chromosome Inactivation
(Molecular Profiling Initiative, NCI)
X chromosome inactivation (methylation) can be used to determine the clonality status of a tumor or premalignant lesion in females. The technique is based on a methylation-sensitive restriction enzyme and analysis of a polymorphic locus on the X chromosome. Clonal cell populations will show "loss" of the non-methylated allele after restriction digest. The assay can be performed on DNA recovered from microdissected samples. Both frozen tissue and fixed-embedded archival tissue can be utilized.
http://cgap-mf.nih.gov/Protocols/DNARNAProteomicAn...
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Comparative Gene Expression Study Using Microarray
(Jeremy Buhler)
http://www.cs.wustl.edu/~jbuhler//research/array/
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Loss of Heterozygosity
(Molecular Profiling Initiative, NCI)
This method is used to detect genomic DNA deletions in tumor cells
http://cgap-mf.nih.gov/Protocols/DNARNAProteomicAn...
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Serial Analysis of Gene Expression
(SAGE)
SAGE is a powerful tool that allows the analysis of overall gene expression patterns with digital analysis. Because SAGE does not require a preexisting clone, it can be used to identify and quantitate new gene as well as know genes.
http://www.sagenet.org/
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