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Protocols
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Hoechst Fluorometric Quantitation of DNA
(Dr. Simon Dawson, Department of Biochemistry, University of Nottingham Medical School)
The concentration of DNA in solutions containing contaminating protein or RNA can be determined quite accurately by measuring the increased fluorescence of the Hoechst dye 33258 upon intercalating into dsDNA.
http://www.nottingham.ac.uk/~mbzspd/methods/Hoechs...
Added: Sat Apr 10 2004, Hits: 1239, Reviews: 0 Write review Cached -
Quantification of Nuclear Acid and Protein
(Brinkmann)
Some basics about nuclear acid and protein quantification by spectrophotometer.
http://www.brinkmann.com/quant_easy.asp
Added: Tue May 14 2002, Hits: 3260, Reviews: 0 Write review Cached -
Quantitation of DNA for Sequencing Using Fluorometer
(Hancock Lab)
(Accessible only by IE)
http://www.cmdr.ubc.ca/bobh/methods/QUANTITATIONOF...
Added: Tue May 14 2002, Hits: 1115, Reviews: 0 Write review Cached -
Quantitation of Nucleic Acid Concentration
(Faulkner-Jones)
Three methods are provided for nucleic acid quantitation: optical density measurements; ethidium bromide - agarose plates, and agarose gel electrophoresis estimation with a known standard DNA.
http://grimwade.biochem.unimelb.edu.au/bfjones/gen...
Added: Tue May 14 2002, Hits: 3490, Reviews: 1 Read reviews Write review Cached -
Spot Test
(Schneitz Lab)
This technique provides a rapid way to make a rough but useful estimate of the DNA concentration of a given sample (e.g. isolated fragment).
http://www.unizh.ch/botinst/Cyto_Website/schneitzL...
Added: Tue May 14 2002, Hits: 1148, Reviews: 0 Write review Cached