Rapid Silver staining |
| Author: | N.Sivaramaiah and krishan Mohan |
|
| Source: | Birla Institute of Scientific Research, Jaipur, India |
| Date Added: | Mon Aug 22 2005 |
| Date Modified: | Wed Aug 24 2005 |
|
Abstract: Rapid staining protocol for SDS PAGE gels
- Fixation (30% Methanol and 5% Acetic acid): Add 50ml of fixing solution
for 10 min
- Water washes: Several changes of water for at least half an hour
- Hypo (10mg/100ml Sodium thiosulfate): Add Hypo for one min and quickly
wash the gel with water
- Silver nitrate (0.1 gm with 100 µl Formaldehyde): Add 50 ml of the
silver nitrate for 8-10 min
- Developer (1.5% with 50 µl Formaldehyde): Quickly wash the gel with
developer to remove the excess stain and gently rinse the gel in Developer
until bands appear
- Stop solution (2M citric acid): Add 5 ml of stop solution
