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Protocols
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Preparation of RNA from Cultured ES Cells
(Allan Bradley's Lab, Baylor College of Medicine, Houston, Texas)
http://www.imgen.bcm.tmc.edu/molgen/labs/bradley/r...
Added: Sun Jul 28 2002, Hits: 314, Reviews: 0 Write review Cached -
Production of Completely ES Cell-Derived Fetuses by Aggragation with Tetraploid Embryos
(Nagy Lab)
http://www.mshri.on.ca/nagy/Tetraploid/Tetra.htm
Added: Tue May 14 2002, Hits: 320, Reviews: 0 Write review Cached -
Production of ES Cell Chimeras by Aggregation wtih Eight-Cell Stage Embryos
(Nagy Lab)
To produce completely ES cell-derived embryos a clump of ES cells is sandwiched between two tetraploid embryos. For ES cell aggregation chimeras with diploid embryos, one embryo is aggregated with a clump of ES cells. Even in this case, the ES cell internalization is efficient enough to achieve a high level of ES cell contribution.
http://www.mshri.on.ca/nagy/diploid/diploid.htm
Added: Tue May 14 2002, Hits: 349, Reviews: 0 Write review Cached -
Rat Cortical Stem Cell Culture Protocol
(R&D System)
http://www.rndsystems.com/stem_cell_protocol_detai...
Added: Sun Mar 02 2008, Hits: 36, Reviews: 0 Write review -
Routine Culturing of ES Cells
(Bowtell Lab)
http://grimwade.biochem.unimelb.edu.au/bowtell/cel...
Added: Tue May 14 2002, Hits: 572, Reviews: 0 Write review Cached -
Routine Splitting and Freezing of Cells
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
http://www.pmci.unimelb.edu.au/core_facilities/man...
Added: Tue May 14 2002, Hits: 365, Reviews: 0 Write review Cached -
Troubleshooting of ES Cell Culture
(Chemicon)
Gives common causes of ES cell differentiation and recommendations on how to help prevent differentiation occurring. Also listed are a number of reasons that often cause the slow growth of ES cell clones, and some causes for the lack of generating chimeras from the cell culture aspect.
http://www.esgro-lif.com/esgro/troubleshooting.htm
Added: Wed Mar 22 2006, Hits: 540, Reviews: 0 Write review Cached