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Protocols
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ES Cell Culture Protocol
(The Wellcome Trust Sanger Institute)
This protocol describes procedures including ES cell thawing, passaging, expanding and freezing.
http://www.sanger.ac.uk/PostGenomics/genetrap/prot...
Added: Wed Jan 30 2008, Hits: 251, Reviews: 0 Write review -
ES Cell Freezing and Thawing
(Nagy Lab)
http://www.mshri.on.ca/nagy/Protocols/esfriz.htm
Added: Tue May 14 2002, Hits: 880, Reviews: 0 Write review Cached -
ES Cell Subcloning Protocol
(NIH Stem Cell Unit)
A feeder-free protocol
http://stemcells.nih.gov/research/NIHresearch/scun...
Added: Fri Oct 07 2005, Hits: 471, Reviews: 0 Write review Cached -
ES-cell injection into mouse blastocysts
(Practical Approach Online, Oxford University Press)
Step by step guide for ES cell microinjection, required reagents and equipment.
http://www.oup.co.uk/pdf/pas/13-10-4.pdf
Added: Wed Sep 04 2002, Hits: 584, Reviews: 0 Write review -
Freezing Embryonic Stem (ES) Cell Clones in 96-Well Plates
(Allan Bradley's Lab, Baylor College of Medicine, Houston, Texas)
http://www.imgen.bcm.tmc.edu/molgen/labs/bradley/f...
Added: Sun Jul 28 2002, Hits: 387, Reviews: 0 Write review Cached -
Guidelines for Culturing ES and MEF Feeder Cells
(Transgenic Research Center, Stanford University)
Routine maintenance of ES cells, culturing feeder cells, cell freezing and thawing.
http://med.stanford.edu/transgenic/esmeflif.html
Added: Wed Mar 22 2006, Hits: 727, Reviews: 0 Write review Cached -
Injection of ES cells into morulae
(Practical Approach Online, Oxford University Press)
http://www.oup.co.uk/pdf/pas/13-10-5.pdf
Added: Wed Sep 04 2002, Hits: 283, Reviews: 0 Write review -
Methods for Expansion of Human Embryonic Stem Cells
(Stem Cells)
This article describes both mechanical and enzymatic transfer methods for human embryonic stem cells (hESCs) depending on experimental purpose.
http://stemcells.alphamedpress.org/cgi/content/ful...
Added: Wed Feb 27 2008, Hits: 58, Reviews: 0 Write review -
Picking ES Cell Colonies and Transferring Them to 24-Well Culture Dishes
(Allan Bradley's Lab, Baylor College of Medicine, Houston, Texas)
http://www.imgen.bcm.tmc.edu/molgen/labs/bradley/p...
Added: Sun Jul 28 2002, Hits: 393, Reviews: 0 Write review Cached -
Preparation of ES cells for Microinjection
(Practical Approach Online, Oxford University Press)
http://www.oup.co.uk/pdf/pas/13-10-3.pdf
Added: Wed Sep 04 2002, Hits: 365, Reviews: 0 Write review