Subcategories

Protocols

• Analysis of ES Cell Clones by Mini-Southern (Allan Bradley's Lab, Baylor College of Medicine, Houston, Texas)
  Step by step procedures including initial cloning, duplicate preparation, mini-southern.
  http://www.imgen.bcm.tmc.edu/molgen/labs/bradley/m...
  Added: Sun Jul 28 2002, Hits: 917, Reviews: 0  Write review   Cached
• b-Galactosidase Color Assay for Cultured Cells (Allan Bradley's Lab, Baylor College of Medicine, Houston, Texas)
  http://www.imgen.bcm.tmc.edu/molgen/labs/bradley/b...
  Added: Sun Jul 28 2002, Hits: 764, Reviews: 0  Write review   Cached
• FACS Analysis of ES Cells (NIH Stem Cell Information)
  FACS analysis for ES cell markers
  http://stemcells.nih.gov/research/NIHresearch/scun...
  Added: Wed Jan 30 2008, Hits: 187, Reviews: 0  Write review   Cached
• Flow Cytometry of Embryonic Stem Cells (ATCC)
  For characterization, a single cell suspension of the cell line in question is analyzed for the presence of specific antibody markers by flow cytometry. The markers were selected from a pool of antibodies based on their availability and their specificity to undifferentiated stem cells and the differentiated cells in each germ layer (ectoderm, mesoderm, and endoderm).
  http://www.atcc.org/common/catalog/cellBiology/ste...
  Added: Mon Dec 31 2007, Hits: 176, Reviews: 0  Write review  
• Immunocytochemistry of Embryonic Stem Cells (ATCC)
  For characterization, cells cultured in vitro on glass coverslips are analyzed for the presence of specific antibody markers by immunocytochemistry.
  http://www.atcc.org/common/catalog/cellBiology/ste...
  Added: Mon Dec 31 2007, Hits: 164, Reviews: 0  Write review  
• Immunostaining of ES Cells (NIH Stem Cell Information)
  Protocol for immunostaining of ES cell markers such as SSEA1, SSEA4, Oct4 and Nanog.
  http://stemcells.nih.gov/research/NIHresearch/scun...
  Added: Wed Jan 30 2008, Hits: 209, Reviews: 0  Write review   Cached
• Karyotyping ES Cells (Bowtell Lab)
  
  http://grimwade.biochem.unimelb.edu.au/bowtell/cel...
  Added: Tue May 14 2002, Hits: 450, Reviews: 0  Write review   Cached
• Karyotyping of ES Cells (millipore)
  http://www.millipore.com/techpublications/tech1/mc...
  Added: Sat Feb 02 2008, Hits: 176, Reviews: 0  Write review   Cached
• RT-PCR of Undifferentiated Cells (NIH Stem Cell Information)
  RT-PCR for stem cell marker genes including primer sequences for human Oct4, Nanog and beta-actin.
  http://stemcells.nih.gov/research/NIHresearch/scun...
  Added: Wed Jan 30 2008, Hits: 130, Reviews: 0  Write review   Cached