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  • Video protocol Hanging Drop Culture for Embryoid Body (EB) Formation (Journal of Visualized Experiments )
    This video protocol demonstrates how to do handing drop culture of ES stem cells.
    http://www.jove.com/index/Details.stp?ID=162
    Added: Mon Feb 25 2008, Hits: 455, Reviews: 0  Write review   Cached
  • EB Differentiation (NIH Stem Cell Information)
    Protocol for testing pluripotency of ES cells
    http://stemcells.nih.gov/research/NIHresearch/scun...
    Added: Wed Jan 30 2008, Hits: 397, Reviews: 0  Write review   Cached
  • Embryoid Body Formation (Thomson Lab, University of Wisconsin)
    Protocol for human ES cell embryoid body formation
    http://ink.primate.wisc.edu/~thomson/protocol.html...
    Added: Mon Dec 31 2007, Hits: 361, Reviews: 0  Write review  
  • Embryoid Body Formation PDF file (ATCC)
    Embryoid Body (EB) culture is used to examine the differentiation potential of the embryonic stem (ES) cell line. The cells are grown using low-attachment dishes in the presence of complete growth medium lacking leukemia inhibitory factor (LIF). This process induces differentiation, permits suspension culture, and causes the cells to form aggregates. These aggregates are allowed to grow for several days or weeks and samples may be taken at various time points for analysis by flow cytometry or antibody staining. The following protocol was established for the formation of EBs from mouse embryonic stem cells.
    http://www.atcc.org/common/catalog/cellBiology/ste...
    Added: Mon Dec 31 2007, Hits: 445, Reviews: 0  Write review  
  • Embryoid Body Formation Protocol PDF file (Invitrogen)
    http://www.invitrogen.com/downloads/Embryoid_Body_...
    Added: Mon Dec 31 2007, Hits: 380, Reviews: 0  Write review  
  • Immunocytochemistry of Embryoid Bodies PDF file (ATCC)
    For characterization, embryoid bodies (EBs) cultured in vitro are analyzed for the presence of specific antibody markers by immunocytochemistry. The markers were selected from a pool of antibodies based on their availability and their specificity to undifferentiated stem cells and the differentiated cells in each germ layer (ectoderm, mesoderm, and endoderm). The antibodies in the following protocol are used to characterize mouse embryonic stem cells (ESC). Other antibodies are recommended to analyze human embryonic stem cells.
    http://www.atcc.org/common/catalog/cellBiology/ste...
    Added: Mon Dec 31 2007, Hits: 252, Reviews: 0  Write review