Overview: Alkaline phosphatase is a stem cell membrane marker and elevated expression of this enzyme is associated with undifferentiated pluripotent stem cell. All primate pluripotent stem cells, like Embryonic stem (ES), embryonic germ (EG) and embryonal carcinoma (EC) cells, express alkaline phosphatase activity. There are different methods of detecting AP such as methods based on enzymatic reaction followed by colorimetric or fast red violet dye, fluorescent detection and immunostaining.
Protocols
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Alkaline Phosphatase (AP) Staining
(Miller lab, Fred Hutchinson Cancer Research Center)
This protocol is for detection of human placental alkaline phosphatase (AP) in cultured cells.
http://www.fhcrc.org/science/labs/miller/hpapstain...
Added: Sat Feb 23 2008, Hits: 370, Reviews: 0 Write review Cached -
Alkaline phosphatase Staining
(Misato Fujita, Tokyo Institute of Technology)
Protocol for medaka embryos
http://www.shigen.nig.ac.jp/medaka/medakabook/inde...
Added: Sat Feb 23 2008, Hits: 274, Reviews: 0 Write review Cached -
Alkaline Phosphatase Staining of ES Cells
(Millipore)
This protocol describes the use of the Alkaline Phosphatase Detection Kit for the phenotypic assessment of ES cell differentiation and has general uses.
http://www.millipore.com/cellbiology/cb3/generalpr...
Added: Sat Feb 23 2008, Hits: 356, Reviews: 0 Write review Cached -
Burstone's Staining Protocol
(IHCWorld)
Burstone's Staining Protocol for Alkaline Phosphatase on Frozen Tissue Sections
http://www.ihcworld.com/_protocols/special_stains/...
Added: Sat Feb 23 2008, Hits: 98, Reviews: 0 Write review Cached -
Human Alkaline Phosphatase Staining
(Nagy Lab, Mount Sinai Hospital)
Human Alkaline Phosphatase staining of transgenic embryo/tissue
http://www.mshri.on.ca/nagy/Protocols/APwmstain.ht...
Added: Sat Feb 23 2008, Hits: 202, Reviews: 0 Write review Cached