Summary: Alkaline phosphatase is a stem cell membrane marker and elevated expression of this enzyme is associated with undifferentiated pluripotent stem cell. All primate pluripotent stem cells, like Embryonic stem (ES), embryonic germ (EG) and embryonal carcinoma (EC) cells, express alkaline phosphatase activity. There are different methods of detecting AP such as methods based on enzymatic reaction followed by colorimetric or fast red violet dye, fluorescent detection and immunostaining.
- Human Alkaline Phosphatase Stain (Miller lab, Fred Hutchinson Cancer Research Center)
This protocol is for detection of human placental alkaline phosphatase (AP) in cultured cells.
Added: Wed Feb 04 2009, Hits: 3287, Reviews: 0
Alkaline phosphatase Staining
(Misato Fujita, Tokyo Institute of Technology)
Protocol for medaka embryos AP staining
Added: Wed Feb 04 2009, Hits: 2504, Reviews: 0
Alkaline Phosphatase Staining of ES Cells
This protocol describes the use of the Alkaline Phosphatase Detection Kit for the phenotypic assessment of ES cell
Added: Wed Feb 04 2009, Hits: 3081, Reviews: 0
Human Alkaline Phosphatase Staining
(Nagy Lab, Mount Sinai Hospital )
Human Alkaline Phosphatase staining of transgenic embryo/tissue
Added: Wed Feb 04 2009, Hits: 1833, Reviews: 0