HUMAN EMBRYONIC STEM CELL PROTOCOLS(NIH Stem Cell Information) A collection of stem cell culture protocols including the followings: General Notes on hESC Culture, Isolation of Primary Mouse Embryo Fibroblasts ,Thawing and preparing p1 MEF feeder plates, Preparation of MEF- Conditioned Medium (MEF-CM), Microdissection Passaging of hESCs, Bulk passaging of hESC , Cryopreservation of hESCs, Thawing of hESCs, Karyotyping, Media Recipes and Solutions, Tissue Culture Flasks and Plates, Reagents and suppliers. http://stemcells.nih.gov/staticresources/research/... Added: Wed Jan 28 2009, Hits: 3016, Reviews: 0Write review
Thomson Lab Stem Cell Protocols(Thomson Lab, University of Wisconsin) A collection of stem cell protocols from Thomson lab including: Human and mouse embryonic stem cell Protocols and iPS cell protocols.
http://ink.primate.wisc.edu/~thomson/protocol.html Added: Wed Jan 28 2009, Hits: 2256, Reviews: 0Write reviewCached
Generating Induced Pluripotent Stem Cells in Mouse Embryonic Fibroblast Cells
(Jaenisch Lab, Whitehead Institute) Pluripotency can be induced in differentiated murine by viral transduction of Oct4, Sox2, Klf4, and c-Myc. We have devised a reprogramming strategy in which these four transcription factors are expressed from doxycycline (dox)-inducible lentiviral vectors. Using these inducible constructs, we can derive induced pluripotent stem (iPS) cells from mouse embryonic fibroblasts (MEFs). In this video, we demonstrate the procedure for the generation of inducible lentiviruses that express the four transcription factors and show how to infect MEFs with these viruses in order to produce iPS cells. By using inducible lentiviruses, the expression of the four factors in controlled by the addition of doxycyline to the culture medium. The advantage of this system over the traditional retroviral infection is the ability to turn the genes on and off so that the kinetics of reprogramming and gene expression requirements can be analyzed in detail. http://www.jove.com/index/details.stp?ID=734 Added: Mon Jul 06 2009, Hits: 1564, Reviews: 0Write reviewCached
Stem Cell Basics
(NIH Stem Cell Information) Provides basic background information about stem cells. http://stemcells.nih.gov/info/basics/Pages/Default... Added: Wed Jan 28 2009, Hits: 1861, Reviews: 0Write reviewCached