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  • Human Embryonic Stem Cell Culture Protocols PDF file (UNIVERSITY OF MINNESOTA)
    Manual for Essential Human Embryonic Stem Cell Culture Methods Course
    http://www.stemcell.umn.edu/img/assets/10061/UMN_h...
    Added: Sat Feb 02 2008, Hits: 110, Reviews: 0  Write review  
  • Theory, principles and background info Stem Cell Basics (NIH)
    covers basic information about stem cells.
    http://stemcells.nih.gov/info/basics/
    Added: Sun Mar 12 2006, Hits: 1195, Reviews: 0  Write review   Cached
  • Stem Cell Protocols (Thomson Lab, University of Wisconsin)

    Collection of basic ES cell manipulation protocols including:

    • Media and reagents
    • Thawing ES Cells
    • Splitting ES Cells on MEF's (stem cell photos)
    • Freezing ES Cells
    • Matrigel Aliquoting and Plating
    • Splitting ES Cells on Matrigel
    • Embryonic Bodies

    http://ink.primate.wisc.edu/~thomson/protocol.html
    Added: Fri Oct 07 2005, Hits: 2352, Reviews: 0  Write review   Cached
  • Human Embryonic Stem Cell Protocols PDF file (BRESAGEN IINC.)
    The following procedures are described:
    • General Notes on hES Culture
    • Isolation of Primary Mouse Embryo Fibroblasts
    • Thawing and preparing p1 MEF feeder plates
    • Preparation of MEF-Conditioned Medium (MEF-CM)
    • Microdissection Passaging of hESCs
    • Bulk passaging of hESC
    • Cryopreservation of hESCs
    • Karyotyping
    • Cell Shipments of cryopreserved vials and T25 flask
    • Media Recipes and Solutions

    http://stemcells.nih.gov/staticresources/research/...
    Added: Mon Feb 27 2006, Hits: 943, Reviews: 0  Write review  
  • Neural Stem Cell Harvest Protocol (Dr. Schwartz, National Human Neural Stem Cell Resource)
    Obtaining stem cells from the brain
    http://www.nhnscr.org/assist/sc_protocol.htm
    Added: Mon Feb 27 2006, Hits: 752, Reviews: 0  Write review   Cached
  • Rat Cortical Stem Cell Culture Protocol (R&D Systems)
    Expansion using monolayer system and neurosphere system, thawing of cryopreserved cells.
    http://www.rndsystems.com/stem_cell_protocol_detai...
    Added: Mon Feb 27 2006, Hits: 666, Reviews: 0  Write review   Cached
  • Tissue Processing and Storage for Stem Cell Isolation (Dr. Schwartz, National Human Neural Stem Cell Resource)
    Including brain, liver, muscle and skin
    http://www.nhnscr.org/assist/tissue_protocol.htm
    Added: Mon Feb 27 2006, Hits: 748, Reviews: 0  Write review   Cached