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Simultaneous analysis of DNA content and surface immunophenotype using gentle ethanol fixation
(Cytomety Lab, Purdue Univ)
This procedure utilizes gentle ethanol treatment techniques, which permeablize cells with minimal loss of surface antigen expression and antibody-antigen association.
http://www.cyto.purdue.edu/flowcyt/research/cytote...
Added: Tue May 14 2002, Hits: 1204, Reviews: 0 Write review Cached -
Simultaneous Measurement of Apoptosis and Necrosis
(The Janis V. Giorgi Flow Cytometry Laboratory, UCLA)
Measurement of apoptosis and necrosis by dual leaser flow cytometry.
http://cyto.mednet.ucla.edu/Protocols/simultta.htm
Added: Sun Feb 12 2006, Hits: 1150, Reviews: 0 Write review Cached -
Staining Procedure for Flow Cytometric Detection of Human Cyclins
(BD PharMingen)
This is a standard protocol used at Pharmingen for Quality Control testing of the anti-cyclin antibodies by flow cytometry. It is a good starting point. However, investigators may need to optimize protocols for their own experimental system. It is particularly useful to refer to the published literature regarding protocols typically used for a given type of protein.
http://www.bdbiosciences.com/pharmingen/protocols/...
Added: Wed Aug 28 2002, Hits: 1523, Reviews: 0 Write review Cached -
Surface Antigen Staining and DNA Content Analysis
(The Janis V. Giorgi Flow Cytometry Laboratory, UCLA)
For correlating surface antigen expression with DNA content
http://cyto.mednet.ucla.edu/Protocols/surface1.htm
Added: Sat Feb 11 2006, Hits: 620, Reviews: 0 Write review Cached -
Yeast Cell Cycle Analysis by Flow Cytometry
(Susan Forsburg)
http://pingu.salk.edu/flow/protocols/ycc.html
Added: Tue May 14 2002, Hits: 627, Reviews: 0 Write review Cached