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Flow Cytometry Protocol
(Sigma)
Covers procedures including Preparation of solutions, Cell culture, Preparation of Samples for DNA analysis, Acquisition of Flow Cytometric DNA Histograms, Analysis of Flow Cytometric DNA Histogram.
https://www.sigmaaldrich.com/Area_of_Interest/Life...
Added: Tue Mar 13 2007, Hits: 849, Reviews: 0 Write review -
Immunodetection of cyclin D1 and D2/D3 using 488/630 nm
( )
dual laser flow cytometry(Cytomety Lab, Purdue Univ)
This protocol is for use with the D cyclins and employs 488 nm argon laser excitation of propidium iodide and 630 nm NeNe or diode laser excitation of the fluorochrome Cy5 to detect cell cycle-specific cyclin D expression
http://www.cyto.purdue.edu/flowcyt/research/cytote...
Added: Tue May 14 2002, Hits: 907, Reviews: 0 Write review Cached -
Immunofluorescence Staining and Flow Cytometry of Intracellular Cytokines
(eBioscience)
A modification of the basic immunofluorescence staining and flow cytometric analysis protocol can be used for the simultaneous analysis of surface molecules and intracellular cytokines at single-cell level. In this protocol, cells are first activated in vitro, stained for surface antigens, as in the regular staining protocol, then fixed and permeabilized to allow for anti-cytokine antibodies to stain intracellularly.
http://www.ebioscience.com/ebioscience/appls/FCI.h...
Added: Tue May 14 2002, Hits: 3433, Reviews: 0 Write review Cached -
Immunofluorescence Staining of Human Cells by Lysed Whole Blood Method
(BD PharMingen)
Standard protocol
http://www.bdbiosciences.com/pharmingen/protocols/...
Added: Wed Aug 28 2002, Hits: 2377, Reviews: 0 Write review Cached -
Immunofluorescent Staining of Mouse and Rat Leukocytes
(BD PharMingen)
Standard protocol including detailed procedures and tips
http://www.bdbiosciences.com/pharmingen/protocols/...
Added: Wed Aug 28 2002, Hits: 2445, Reviews: 0 Write review Cached -
Indirect Immunofluorescence Staining
(U of Florida Flow Cytometry Core Laboratory)
This is the method for indirect immunofluorescence labeling; that is, the antibodies do not have the fluorescent dye attached.
http://www.biotech.ufl.edu/FlowCytometry/indirect....
Added: Tue May 14 2002, Hits: 1421, Reviews: 0 Write review Cached -
Paraformaldehyde preparation
( )
http://pingu.salk.edu/flow/protocols/pfm.html
Added: Tue May 14 2002, Hits: 2107, Reviews: 1 Read reviews Write review Cached -
Phenotype-specific immunodetection of cyclins using 488/630 nm dual laser flow cytometry
(Cytomety Lab, Purdue Univ)
This protocol is for use with the D and E cyclins and employs 488 nm argon laser excitation of propidium iodide and a FITC-conjugated phenotypic label, and 630 nm NeNe or diode laser excitation of the fluorochrome Cy5 to detect cell cycle-specific cyclin D expression.
http://www.cyto.purdue.edu/flowcyt/research/cytote...
Added: Tue May 14 2002, Hits: 546, Reviews: 0 Write review Cached -
Phycoerythrin Labeling
(David Chambers)
http://pingu.salk.edu/flow/protocols/pelabel.html
Added: Tue May 14 2002, Hits: 509, Reviews: 0 Write review Cached -
Protein (Antibody) Fluorescent Labeling
(Mario Roederer)
Gives detailed protocol for conjugation of proteins (principally monoclonal antibodies) to fluorescent dyes. The conjugations fall into four basic protocols: Type 1 (used for FITC, Cy5, and the initial preparation of the Cy5 and Cy7 tandem dyes); Type 2 (used for Biotin and Cascade Blue); Type 3 (used for conjugation of PE, APC, TR-BSA, and their derivatives); and Type 4 (used for the initial preparation of non-immunoglobulin proteins like Annexin V).
http://www.drmr.com/abcon/
Added: Tue May 14 2002, Hits: 1267, Reviews: 0 Write review Cached