Protocols
- Simultaneous Cell Surface, BrdU and DNA Content Staining for Flow Cytometry (Fero Lab, Fred Hutchinson Cancer Research Center)
An assay for the simultaneous measurement of cell surface phenotype, S-phase fraction, and DNA content by single laser instrumentation for the purpose of determining the labeling index (LI), duration of S-phase (Ts), and the potential doubling time (Tpot)
http://labs.fhcrc.org/fero/Protocols/CellSurfaceBr...
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Immunodetection of cyclin D1 and D2/D3 using 488/630 nm
( )
dual laser flow cytometry(Cytomety Lab, Purdue Univ)
This protocol is for use with the D cyclins and employs 488 nm argon laser excitation of propidium iodide and 630 nm NeNe or diode laser excitation of the fluorochrome Cy5 to detect cell cycle-specific cyclin D expression
http://www.cyto.purdue.edu/flowcyt/research/cytote...
Added: Tue May 14 2002, Hits: 1021, Reviews: 0 Write review Cached -
Immunofluorescence Staining and Flow Cytometry of Intracellular Cytokines
(eBioscience)
A modification of the basic immunofluorescence staining and flow cytometric analysis protocol can be used for the simultaneous analysis of surface molecules and intracellular cytokines at single-cell level. In this protocol, cells are first activated in vitro, stained for surface antigens, as in the regular staining protocol, then fixed and permeabilized to allow for anti-cytokine antibodies to stain intracellularly.
http://www.ebioscience.com/ebioscience/appls/FCI.h...
Added: Tue May 14 2002, Hits: 2761, Reviews: 0 Write review Cached -
Measurement of Intracellular CA2+ and Cell Surface Antigen Expression
(UCLA Flow Cytometry Core)
http://cyto.mednet.ucla.edu/Protocols/Calcium%20St...
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Nuclear Antigens and DNA Content with PI Staining
(UCSD Cancer Center Flow Cytometry Core Facility)
Stain cells for a nuclear antigen and DNA content
http://cancer.ucsd.edu/flow/Protocols/DNA&NuclearA...
Added: Sun Aug 01 2010, Hits: 201, Reviews: 0 Write review Cached -
Phenotype-specific immunodetection of cyclins using 488/630 nm dual laser flow cytometry
(Cytomety Lab, Purdue Univ)
This protocol is for use with the D and E cyclins and employs 488 nm argon laser excitation of propidium iodide and a FITC-conjugated phenotypic label, and 630 nm NeNe or diode laser excitation of the fluorochrome Cy5 to detect cell cycle-specific cyclin D expression.
http://www.cyto.purdue.edu/flowcyt/research/cytote...
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Simultaneous analysis of DNA content and surface immunophenotype using gentle ethanol fixation
(Cytomety Lab, Purdue Univ)
This procedure utilizes gentle ethanol treatment techniques, which permeablize cells with minimal loss of surface antigen expression and antibody-antigen association.
http://www.cyto.purdue.edu/flowcyt/research/cytote...
Added: Tue May 14 2002, Hits: 981, Reviews: 0 Write review Cached -
Simultaneous Measurement of Apoptosis and Necrosis
(The Janis V. Giorgi Flow Cytometry Laboratory, UCLA)
Simultaneous Measurement of Apoptosis and Necrosis
http://cyto.mednet.ucla.edu/Protocols/simultta.htm
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Simultaneous Measurement of Cell Surface Immunofluorescence, Viability, and DNA Content
(UCLA Flow Cytometry Core)
http://cyto.mednet.ucla.edu/Protocols/Simultaneous...
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Simultaneous Measurement of Dual-Color Immunofluorescence and DNA/RNA Content
(UCLA Flow Cytometry Core)
http://cyto.mednet.ucla.edu/Protocols/2%20color%20...
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Simultaneous Measurement of Dual-Color Immunofluorescence and DNA/RNA Content
(UCLA Flow Cytometry Core)
http://cyto.mednet.ucla.edu/Protocols/2%20color%20...
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Simultaneous Measurement of Three-Color Immunofluorescence and DNA Content
(UCLA Flow Cytometry Core)
http://cyto.mednet.ucla.edu/Protocols/3color%20DNA...
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Surface Antigen and DNA Content with PI Staining
(UCSD Cancer Center Flow Cytometry Core Facility)
To stain surface antigens and measure DNA Content simultaneously.
http://cancer.ucsd.edu/flow/Protocols/HypoDNA.asp
Added: Sun Aug 01 2010, Hits: 206, Reviews: 0 Write review Cached -
Surface Antigen Staining and DNA Content
(UCLA Flow Cytometry Core)
http://cyto.mednet.ucla.edu/Protocols/surface1.htm
Added: Sat Jan 31 2009, Hits: 796, Reviews: 0 Write review Cached