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Protocols

• Simultaneous Cell Surface, BrdU and DNA Content Staining for Flow Cytometry (Fero Lab, Fred Hutchinson Cancer Research Center)
  An assay for the simultaneous measurement of cell surface phenotype, S-phase fraction, and DNA content by single laser instrumentation for the purpose of determining the labeling index (LI), duration of S-phase (Ts), and the potential doubling time (Tpot)
  http://labs.fhcrc.org/fero/Protocols/CellSurfaceBr...
  Added: Sat Jan 31 2009, Hits: 1153, Reviews: 0  Write review   Cached
• Immunodetection of cyclin D1 and D2/D3 using 488/630 nm ( )
  dual laser flow cytometry(Cytomety Lab, Purdue Univ)
  This protocol is for use with the D cyclins and employs 488 nm argon laser excitation of propidium iodide and 630 nm NeNe or diode laser excitation of the fluorochrome Cy5 to detect cell cycle-specific cyclin D expression
  http://www.cyto.purdue.edu/flowcyt/research/cytote...
  Added: Tue May 14 2002, Hits: 650, Reviews: 0  Write review   Cached
• Immunofluorescence Staining and Flow Cytometry of Intracellular Cytokines (eBioscience)
  A modification of the basic immunofluorescence staining and flow cytometric analysis protocol can be used for the simultaneous analysis of surface molecules and intracellular cytokines at single-cell level. In this protocol, cells are first activated in vitro, stained for surface antigens, as in the regular staining protocol, then fixed and permeabilized to allow for anti-cytokine antibodies to stain intracellularly.
  http://www.ebioscience.com/ebioscience/appls/FCI.h...
  Added: Tue May 14 2002, Hits: 1840, Reviews: 0  Write review   Cached
• Measurement of Intracellular CA2+ and Cell Surface Antigen Expression (UCLA Flow Cytometry Core)
  http://cyto.mednet.ucla.edu/Protocols/Calcium%20St...
  Added: Sat Jan 31 2009, Hits: 446, Reviews: 0  Write review   Cached
• Nuclear Antigens and DNA Content with PI Staining (UCSD Cancer Center Flow Cytometry Core Facility)
  Stain cells for a nuclear antigen and DNA content
  http://cancer.ucsd.edu/flow/Protocols/DNA&NuclearA...
  Added: Sun Aug 01 2010, Hits: 130, Reviews: 0  Write review   Cached
• Phenotype-specific immunodetection of cyclins using 488/630 nm dual laser flow cytometry (Cytomety Lab, Purdue Univ)
  This protocol is for use with the D and E cyclins and employs 488 nm argon laser excitation of propidium iodide and a FITC-conjugated phenotypic label, and 630 nm NeNe or diode laser excitation of the fluorochrome Cy5 to detect cell cycle-specific cyclin D expression.
  http://www.cyto.purdue.edu/flowcyt/research/cytote...
  Added: Tue May 14 2002, Hits: 458, Reviews: 0  Write review   Cached
• Simultaneous analysis of DNA content and surface immunophenotype using gentle ethanol fixation (Cytomety Lab, Purdue Univ)
  This procedure utilizes gentle ethanol treatment techniques, which permeablize cells with minimal loss of surface antigen expression and antibody-antigen association.
  http://www.cyto.purdue.edu/flowcyt/research/cytote...
  Added: Tue May 14 2002, Hits: 605, Reviews: 0  Write review   Cached
• Simultaneous Measurement of Apoptosis and Necrosis (The Janis V. Giorgi Flow Cytometry Laboratory, UCLA)
  Simultaneous Measurement of Apoptosis and Necrosis
  http://cyto.mednet.ucla.edu/Protocols/simultta.htm
  Added: Sat Jan 31 2009, Hits: 446, Reviews: 0  Write review   Cached
• Simultaneous Measurement of Cell Surface Immunofluorescence, Viability, and DNA Content (UCLA Flow Cytometry Core)
  http://cyto.mednet.ucla.edu/Protocols/Simultaneous...
  Added: Sat Jan 31 2009, Hits: 445, Reviews: 0  Write review   Cached
• Simultaneous Measurement of Dual-Color Immunofluorescence and DNA/RNA Content (UCLA Flow Cytometry Core)
  http://cyto.mednet.ucla.edu/Protocols/2%20color%20...
  Added: Sat Jan 31 2009, Hits: 271, Reviews: 0  Write review   Cached
• Simultaneous Measurement of Dual-Color Immunofluorescence and DNA/RNA Content (UCLA Flow Cytometry Core)
  http://cyto.mednet.ucla.edu/Protocols/2%20color%20...
  Added: Sat Jan 31 2009, Hits: 202, Reviews: 0  Write review   Cached
• Simultaneous Measurement of Three-Color Immunofluorescence and DNA Content (UCLA Flow Cytometry Core)
  http://cyto.mednet.ucla.edu/Protocols/3color%20DNA...
  Added: Sat Jan 31 2009, Hits: 321, Reviews: 0  Write review   Cached
• Surface Antigen and DNA Content with PI Staining (UCSD Cancer Center Flow Cytometry Core Facility)
  To stain surface antigens and measure DNA Content simultaneously.
  http://cancer.ucsd.edu/flow/Protocols/HypoDNA.asp
  Added: Sun Aug 01 2010, Hits: 139, Reviews: 0  Write review   Cached
• Surface Antigen Staining and DNA Content (UCLA Flow Cytometry Core)
  http://cyto.mednet.ucla.edu/Protocols/surface1.htm
  Added: Sat Jan 31 2009, Hits: 440, Reviews: 0  Write review   Cached