Protocols

• Immunodetection of cyclin D1 and D2/D3 using 488/630 nm ( )
  dual laser flow cytometry(Cytomety Lab, Purdue Univ)
  This protocol is for use with the D cyclins and employs 488 nm argon laser excitation of propidium iodide and 630 nm NeNe or diode laser excitation of the fluorochrome Cy5 to detect cell cycle-specific cyclin D expression
  http://www.cyto.purdue.edu/flowcyt/research/cytote...
  Added: Tue May 14 2002, Hits: 929, Reviews: 0  Write review   Cached
• Measurement of Intracellular CA2+ and Cell Surface Antigen Expression (UCLA Flow Cytometry Core)
  http://cyto.mednet.ucla.edu/Protocols/Calcium%20St...
  Added: Thu Jul 10 2008, Hits: 25, Reviews: 0  Write review  
• Simultaneous analysis of DNA content and surface immunophenotype using gentle ethanol fixation (Cytomety Lab, Purdue Univ)
  This procedure utilizes gentle ethanol treatment techniques, which permeablize cells with minimal loss of surface antigen expression and antibody-antigen association.
  http://www.cyto.purdue.edu/flowcyt/research/cytote...
  Added: Tue May 14 2002, Hits: 1239, Reviews: 0  Write review   Cached
• Simultaneous Cell Surface, BrdU and DNA Content Staining for Flow Cytometry (Fero Lab, Fred Hutchinson Cancer Research Center)
  An assay for the simultaneous measurement of cell surface phenotype, S-phase fraction, and DNA content by single laser instrumentation for the purpose of determining the labeling index (LI), duration of S-phase (Ts), and the potential doubling time (Tpot)
  http://www.fhcrc.org/science/labs/fero/Protocols/C...
  Added: Thu Jul 10 2008, Hits: 28, Reviews: 0  Write review  
• Simultaneous Measurement of Apoptosis and Necrosis (The Janis V. Giorgi Flow Cytometry Laboratory, UCLA)
  Measurement of apoptosis and necrosis by dual leaser flow cytometry.
  http://cyto.mednet.ucla.edu/Protocols/simultta.htm
  Added: Sun Feb 12 2006, Hits: 1194, Reviews: 0  Write review   Cached
• Simultaneous Measurement of Cell Surface Immunofluorescence, Viability, and DNA Content (UCLA Flow Cytometry Core)
  http://cyto.mednet.ucla.edu/Protocols/Simultaneous...
  Added: Thu Jul 10 2008, Hits: 33, Reviews: 0  Write review  
• Simultaneous Measurement of Dual-Color Immunofluorescence and DNA/RNA Content (UCLA Flow Cytometry Core)
  http://cyto.mednet.ucla.edu/Protocols/2%20color%20...
  Added: Thu Jul 10 2008, Hits: 17, Reviews: 0  Write review  
• Simultaneous Measurement of Three-Color Immunofluorescence and DNA Content (UCLA Flow Cytometry Core)
  http://cyto.mednet.ucla.edu/Protocols/3color%20DNA...
  Added: Thu Jul 10 2008, Hits: 17, Reviews: 0  Write review  
• Simultaneous Nuclear and Surface Immunostaining for Flow Cytometry (Fero Lab, Fred Hutchinson Cancer Research Center)
  http://www.fhcrc.org/science/labs/fero/Protocols/N...
  Added: Thu Jul 10 2008, Hits: 30, Reviews: 0  Write review  
• Surface Antigen Staining and DNA Content Analysis (The Janis V. Giorgi Flow Cytometry Laboratory, UCLA)
  For correlating surface antigen expression with DNA content
  http://cyto.mednet.ucla.edu/Protocols/surface1.htm
  Added: Sat Feb 11 2006, Hits: 649, Reviews: 0  Write review   Cached