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Protocols
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Metaphase Chromosome Preparation from Mouse/Rat Spleen
(Thomas Ried's Lab, NCI)
http://www.riedlab.nci.nih.gov/publications/2411.3...
Added: Fri Oct 18 2002, Hits: 564, Reviews: 0 Write review -
Metaphase Preparation from Mouse Embryonic Fibroblast (MEF)
(Thomas Ried's Lab, NCI)
http://www.riedlab.nci.nih.gov/publications/2409.3...
Added: Fri Oct 18 2002, Hits: 562, Reviews: 0 Write review -
Mitotic chromosomes from mouse peripheral blood
(Practical Approach Online, Oxford University Press)
http://www.oup.co.uk/pdf/pas/13-6a-1.pdf
Added: Wed Sep 04 2002, Hits: 492, Reviews: 0 Write review -
Pelleting Assembled Chromatin for Microscopy
(Mitchison Lab)
http://mitchison.med.harvard.edu/protocols/chr2.ht...
Added: Tue May 14 2002, Hits: 310, Reviews: 0 Write review Cached -
Q-Banding
(Waisman Center Cytogenetics Lab, University of Wisconsin - Madison)
For the identification of individual chromosomes and their structural anomalies
http://www.slh.wisc.edu/cytogenetics/Protocols/Sta...
Added: Mon Dec 09 2002, Hits: 207, Reviews: 0 Write review Cached -
Replication Banding
(Waisman Center Cytogenetics Lab, University of Wisconsin - Madison)
Provides differential staining of early and late replicating areas of the chromosomes. Allows identification of the early replicating X chromosome. Can be used for marker chromosome analysis. When Bromo deoxyuridine is added at the end of culture late replicating areas will stain pale and early replicating areas will stain dark.
http://www.slh.wisc.edu/cytogenetics/Protocols/Sta...
Added: Mon Dec 09 2002, Hits: 191, Reviews: 0 Write review Cached -
Salivary Gland Preparation (Squash technigue
(Dr. William H. Heidcamp, Biology Department, Gustavus Adolphus College)
http://homepages.gac.edu/~cellab/chpts/chpt10/ex10...
Added: Tue May 14 2002, Hits: 350, Reviews: 0 Write review Cached -
Solid Tumor Cultures for Chromosome Analysis
(Waisman Center Cytogenetics Lab, University of Wisconsin - Madison)
A. Samples of solid tumors or lymph nodes may be sent from patients with cancer. These samples should be processed directly and also set up for short term culture of tumor cells. Care must be taken to avoid over-growth of normal fibroblast cells that are also present in the tumor specimen.
http://www.slh.wisc.edu/cytogenetics/Protocols/Cul...
Added: Mon Dec 09 2002, Hits: 448, Reviews: 0 Write review Cached -
Tissue Fibroblast Cultures for Chromosome Analysis
(Waisman Center Cytogenetics Lab, University of Wisconsin - Madison)
Skin tissue may be used for chromosome analysis in special cases when the results from peripheral blood are inconclusive...
http://www.slh.wisc.edu/cytogenetics/Protocols/Cul...
Added: Mon Dec 09 2002, Hits: 641, Reviews: 0 Write review Cached -
Vital Staining of Chromosomes with Hoechst 33258
(Yu-li Wang's Lab, University of Massachusetts Medical School )
http://ylwang.umassmed.edu/protocol/cfs/hoechst.ht...
Added: Fri Oct 17 2003, Hits: 1355, Reviews: 0 Write review Cached