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Protocols
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Amniotic Fluid Cultures on Coverslips
(Waisman Center Cytogenetics Lab, University of Wisconsin - Madison)
Amniotic fluid may be used for prenatal diagnosis of aneuploidy or other structural abnormalities.
http://www.slh.wisc.edu/cytogenetics/Protocols/Cul...
Added: Mon Dec 09 2002, Hits: 220, Reviews: 0 Write review Cached -
Centrosome Preparation
(Mitchison Lab)
http://mitchison.med.harvard.edu/protocols/mt1.htm...
Added: Tue May 14 2002, Hits: 493, Reviews: 0 Write review Cached -
CHO Chromosome Preparation
(Mitchison Lab)
http://mitchison.med.harvard.edu/protocols/chr1.ht...
Added: Tue May 14 2002, Hits: 539, Reviews: 0 Write review Cached -
Chorionic Villus Tissue for Chromosome Analysis
(Waisman Center Cytogenetics Lab, University of Wisconsin - Madison)
Chorionic villus tissue may be used for prenatal diagnosis of aneuploidy or other structural abnormalities. It is usually done between the 9th and 11th week of gestation. Chorionic villus is not derived from the actual fetus and in rare cases may not have the same karyotype as the fetus, giving false results.
http://www.slh.wisc.edu/cytogenetics/Protocols/Cul...
Added: Mon Dec 09 2002, Hits: 76, Reviews: 0 Write review Cached -
Chromosomal DNA Prep : cultured cells/tissue samples
(Mike A Dyer)
This protocol was developed for cultured cells but should be appropriate for dissociated tissues as well.
http://axon.med.harvard.edu/~cepko/protocol/mike/C...
Added: Tue May 14 2002, Hits: 402, Reviews: 0 Write review Cached -
Distamycin A Plus DAPI Fluorescent Staining (Dst/DAPI)
(Waisman Center Cytogenetics Lab, University of Wisconsin - Madison)
In man, this technique produces brilliant fluorescence at the C-blocks of chromosomes 1, 9, and 16, at the heterochromatic part of the Y, and at a short arm segment of chromosome 15 (15pll).
http://www.slh.wisc.edu/cytogenetics/Protocols/Sta...
Added: Mon Dec 09 2002, Hits: 141, Reviews: 0 Write review Cached -
High Resolution Chromosomes
(Thomas Ried's Lab, NCI)
http://www.riedlab.nci.nih.gov/publications/2407.3...
Added: Fri Oct 18 2002, Hits: 631, Reviews: 0 Write review -
In Vitro Chromatin and Chromosome Assembly
(Mitchison Lab)
http://mitchison.med.harvard.edu/protocols/chr3.ht...
Added: Tue May 14 2002, Hits: 385, Reviews: 0 Write review Cached -
Metaphase Chromosome Preparation from Mouse/Rat Spleen
(Thomas Ried's Lab, NCI)
http://www.riedlab.nci.nih.gov/publications/2411.3...
Added: Fri Oct 18 2002, Hits: 598, Reviews: 0 Write review -
Mitotic chromosomes from mouse peripheral blood
(Practical Approach Online, Oxford University Press)
http://www.oup.co.uk/pdf/pas/13-6a-1.pdf
Added: Wed Sep 04 2002, Hits: 443, Reviews: 0 Write review -
Solid Tumor Cultures for Chromosome Analysis
(Waisman Center Cytogenetics Lab, University of Wisconsin - Madison)
A. Samples of solid tumors or lymph nodes may be sent from patients with cancer. These samples should be processed directly and also set up for short term culture of tumor cells. Care must be taken to avoid over-growth of normal fibroblast cells that are also present in the tumor specimen.
http://www.slh.wisc.edu/cytogenetics/Protocols/Cul...
Added: Mon Dec 09 2002, Hits: 147, Reviews: 0 Write review Cached -
Tissue Fibroblast Cultures for Chromosome Analysis
(Waisman Center Cytogenetics Lab, University of Wisconsin - Madison)
Skin tissue may be used for chromosome analysis in special cases when the results from peripheral blood are inconclusive...
http://www.slh.wisc.edu/cytogenetics/Protocols/Cul...
Added: Mon Dec 09 2002, Hits: 234, Reviews: 0 Write review Cached