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Protocols
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Determination of IC50
(The Science Advisory Board)
In vitro whole cell assay by [H3] Hypoxanthine uptake assay.
http://www.scienceboard.net/resources/protocols.as...
Added: Wed Apr 21 2004, Hits: 1240, Reviews: 0 Write review Cached -
Measurement of Incorporation of 35S-Cysteine into Cellular Protein
(The Science Advisory Board)
This protocol can be used to measure the amount of incorporation of 35S-Cysteine into cellular protein following a metabolic labeling procedure. This protocol reduces the amount of non-specific counts, which can be considerable with labeled Cysteine.
http://www.scienceboard.net/resources/protocols.as...
Added: Sun Jun 05 2005, Hits: 344, Reviews: 0 Write review Cached -
Methods for Cell Death Staining
(Steven Finkbeiner, Departments of Neurology and Physiology, UCSF)
Includes trypan blue staining, propidium iodide staining of living cells and fixed cells, Hoescht staining.
http://gweb1.ucsf.edu/labs/finkbeiner/Protocol/pro...
Added: Tue Oct 01 2002, Hits: 7286, Reviews: 0 Write review Cached -
Proliferation Assay-[3H] Thymidine incorporation
(Hannun/Obeid Lab)
http://www.musc.edu/bcmb/ceramide/protocols/0017.h...
Added: Tue May 14 2002, Hits: 3263, Reviews: 0 Write review Cached -
Radioactive Proliferation Assay
(CellDeath.de)
The Proliferation Assay allows to determine the number of cells that are growing in the absence or presence of certain proliferation affecting agents, e.g. TNF-alpha or anti-Fas antibody (IPO-4).
http://www.celldeath.de/apometh/prolif.html
Added: Tue Jul 17 2007, Hits: 182, Reviews: 0 Write review Cached -
Timing of Cycles
(Dr. William H. Heidcamp, Biology Department, Gustavus Adolphus College)
Determine Cell cycle by exposing cell to 3H-Thymidine, and subsequently fixing and autoradiography.
http://homepages.gac.edu/~cellab/chpts/chpt11/ex11...
Added: Tue May 14 2002, Hits: 633, Reviews: 0 Write review Cached -
[3H]-Thymidine Incorporation in 6-Well Plates
(Hannun/Obeid Lab, Duke Univ.)
For 6-well plate assay
http://www.duke.edu/web/ceramide/protocols/0017.ht...
Added: Sun Apr 24 2005, Hits: 974, Reviews: 0 Write review Cached -
[3H]-Thymidine Uptake by Cultured Cells
(The Science Advisory Board)
Thymidine incorporation assay (TIA) in which cells are grown on coverglasses and then give them H-thymidine for a short period of time...
http://www.scienceboard.net/resources/protocols.as...
Added: Sun Apr 24 2005, Hits: 1205, Reviews: 0 Write review Cached