Subcategories
Protocols
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Amniotic Fluid Cultures on Coverslips
(Waisman Center Cytogenetics Lab, University of Wisconsin - Madison)
Amniotic fluid may be used for prenatal diagnosis of aneuploidy or other structural abnormalities.
http://www.slh.wisc.edu/cytogenetics/Protocols/Cul...
Added: Mon Dec 09 2002, Hits: 220, Reviews: 0 Write review Cached -
Bone Marrow Culture for Chromosome Preparation
(Waisman Center Cytogenetics Lab, University of Wisconsin - Madison)
To identify chromosome anomalies in hematopoietic cells. Used especially for chromosome studies for hematological disorders such as preleukemia and leukemia.
http://www.slh.wisc.edu/cytogenetics/Protocols/Cul...
Added: Mon Dec 09 2002, Hits: 267, Reviews: 0 Write review Cached - Cloning by Limiting Dilution of Hybridoma (Contributed by Nanci Donacki)
Added: Tue May 14 2002, Reviews: 0 Write review -
Collagen gel for raft culture (dermal equivalent)
(Matt Lewis, Department of Pathology, University of Liverpool)
http://www.methodbook.net/cellcult/collgel.html
Added: Fri Sep 06 2002, Hits: 1172, Reviews: 0 Write review Cached -
Culture of BEND cells (a bovine endometrial cell line)
(P.J Hansen Lab)
http://www.animal.ufl.edu/hansen/protocols/bend.ht...
Added: Tue May 14 2002, Hits: 718, Reviews: 0 Write review Cached -
Culture of endometrial explants
(P.J Hansen Lab)
http://www.animal.ufl.edu/hansen/protocols/endocul...
Added: Tue May 14 2002, Hits: 732, Reviews: 0 Write review Cached -
Culturing Chick Embryonic Cardiac Myocytes and Fibroblasts
(Yu-li Wang's Lab, University of Massachusetts Medical School )
Provides materials and detailed procedures
http://ylwang.umassmed.edu/protocol/cc/cardiac.htm
Added: Fri Oct 17 2003, Hits: 472, Reviews: 0 Write review Cached -
Culturing Chick Embryonic Myoblasts/Myotubes and Fibroblasts
(Yu-li Wang's Lab, University of Massachusetts Medical School )
http://ylwang.umassmed.edu/protocol/cc/myotube.htm
Added: Fri Oct 17 2003, Hits: 486, Reviews: 0 Write review Cached -
Culturing HEK 293 Cells
(Dario Neri's labs, Institute of Pharmaceutical Sciences ETHZ)
http://www.pharma.ethz.ch/institute_groups/biomacr...
Added: Mon Sep 09 2002, Hits: 1731, Reviews: 2 Read reviews Write review Cached -
Dissociated rat hippocampus culture (RHC)
(Lab Ibanez-Tallon, Max-Delbrück-Center for Molecular Medicine, Berlin, Germany)
http://www.mdc-berlin.de/en/research/research_team...
Added: Sun Mar 06 2011, Hits: 480, Reviews: 0 Write review -
Elastomeric PGS Scaffolds in Arterial Tissue Engineering
(JoVE)
The objective of this video protocol is to introduce a fabrication technique of porous tubular scaffolds and a dynamic mechanical conditioning for applying them to arterial tissue engineering.
http://www.jove.com/details.php?id=2691
Added: Mon May 16 2011, Hits: 462, Reviews: 0 Write review -
Fibroblast Cell Culture
(Dario Neri's labs, Institute of Pharmaceutical Sciences ETHZ)
This protocol is adapted from Clonetics manual but some procedures may be used for general purposes. Here are some procedures described: Cell isolation, thawing, seeding, subculture, and more.
http://www.pharma.ethz.ch/institute_groups/biomacr...
Added: Mon Sep 09 2002, Hits: 2685, Reviews: 0 Write review Cached -
lacrimal gland cell culture and analysis
(Gordon Laurie, University of Virginia )
Detailed method on lacrimal cell culture and analysis.
http://people.virginia.edu/~gwl6s/home.html/Method...
Added: Mon Jul 29 2002, Hits: 695, Reviews: 0 Write review Cached -
Maintaining Lymphoblastoid Cell Lines
(Donis-Keller lab)
To grow lymphoblastoid cells for permanent storage and for DNA extraction.
http://hg.wustl.edu/hdk_lab_manual/hcc/hcc9.html
Added: Tue May 14 2002, Hits: 681, Reviews: 0 Write review Cached -
Megakaryocyte Culture from Mouse Fetal Livers
(Shivdasani Lab, Harvard Medical School)
The protocol for culturing mouse megakaryocytes. Briefly, whole livers are recovered aseptically from mouse fetuses between embryonic days 13 and 15...
http://research.dfci.harvard.edu/shivdasani/protoc...
Added: Fri Apr 03 2009, Hits: 678, Reviews: 0 Write review -
Micronucleus Assay in Blood Lymphocytes
(Nina T. Holland, Division of Environmental Health Sciences, Berkeley)
Detailed procedures for lymphocyte isolation from blood, lymphocyte culture and micronucleus assay.
http://ehs.sph.berkeley.edu/holland/protocolLibrar...
Added: Wed Oct 02 2002, Hits: 1123, Reviews: 0 Write review Cached -
Organotypic raft culture of primary human keratinocytes (PHKs)
(Matt Lewis, Department of Pathology, University of Liverpool)
http://www.methodbook.net/cellcult/rafting.html
Added: Fri Sep 06 2002, Hits: 832, Reviews: 0 Write review Cached -
PC12 Cell Culture and Fusion
(Yu-li Wang's Lab, University of Massachusetts Medical School )
PC12 pheochromocytoma cells culture, fusion and differentiation.
http://ylwang.umassmed.edu/protocol/cc/pc12.htm
Added: Fri Oct 17 2003, Hits: 772, Reviews: 1 Read reviews Write review Cached -
Preparation of Embryonic Extract
(Yu-li Wang's Lab, University of Massachusetts Medical School )
http://ylwang.umassmed.edu/protocol/cc/embext.htm
Added: Fri Oct 17 2003, Hits: 355, Reviews: 0 Write review Cached -
Serum-Free, High-Density Suspension Culture of 293 Cells
(Dr. Volker Briese, Universitäts-Frauenklinik Rostock)
Manuals from Invitrogen
http://www-ufk.med.uni-rostock.de/lablinks/protoco...
Added: Mon Oct 28 2002, Hits: 225, Reviews: 0 Write review Cached -
Solid Tumor Cultures for Chromosome Analysis
(Waisman Center Cytogenetics Lab, University of Wisconsin - Madison)
A. Samples of solid tumors or lymph nodes may be sent from patients with cancer. These samples should be processed directly and also set up for short term culture of tumor cells. Care must be taken to avoid over-growth of normal fibroblast cells that are also present in the tumor specimen.
http://www.slh.wisc.edu/cytogenetics/Protocols/Cul...
Added: Mon Dec 09 2002, Hits: 147, Reviews: 0 Write review Cached -
Tissue Culture of PtK1 Cells
(Salmon Lab, University of North Carolina at Chapel Hill, Department of Biology)
http://www.bio.unc.edu/faculty/salmon/lab/protocol...
Added: Tue May 14 2002, Hits: 888, Reviews: 0 Write review Cached -
Tissue Culture on Coverslips
(Waisman Center Cytogenetics Lab, University of Wisconsin - Madison)
Skin tissue may be used for chromosome analysis in special cases when the results from peripheral blood are inconclusive, e.g. suspected mosaicism, confirmation of a new chromosome disorder, or special dermatological disorders. Tissue may also be used when blood cultures are not available, such as on stillbirths.
http://www.slh.wisc.edu/cytogenetics/Protocols/Cul...
Added: Mon Dec 09 2002, Hits: 224, Reviews: 0 Write review Cached -
Tissue Fibroblast Cultures for Chromosome Analysis
(Waisman Center Cytogenetics Lab, University of Wisconsin - Madison)
Skin tissue may be used for chromosome analysis in special cases when the results from peripheral blood are inconclusive...
http://www.slh.wisc.edu/cytogenetics/Protocols/Cul...
Added: Mon Dec 09 2002, Hits: 272, Reviews: 0 Write review Cached