Subcategories
Related Categories:
Sponsored Links:
Protocols
-
Cell Culture Guidelines
(Immunology Applications Core Facility, University of Chicago)
General guidelines for culturing cells.
http://iacf.bsd.uchicago.edu/MabHome/Cellculturepr...
Added: Mon Apr 19 2004, Hits: 741, Reviews: 0 Write review Cached -
Conventions for Work in the Cell Culture Laboratory
(Gumbleton Lab, Cardiff University)
General guidelines and good practice for work in cell culture facility. Highly recommended.
http://www.cf.ac.uk/phrmy/staffinfo/MG/LabProtocol...
Added: Wed Feb 11 2009, Hits: 1954, Reviews: 0 Write review -
Growing More Cells: A Simple Guide to Small Volume Cell Culture Scale-Up
(Corning)
Life science researchers are constantly being asked to grow more cells for cell-based experiments and assays, and producing recombinant proteins, antibodies, vaccines and viral vectors. There are a wide variety of products and solutions for scaling up cell cultures to meet these requirements. This 16-page guide will help you select the vessels and techniques that can best meet your needs for growing large amounts of cells (up to 1010cells) or producing more products from these cells in a typical research laboratory setting. * Topics covered include: Advantages and disadvantages of the different vessels and systems used for growing large amounts of cells * How to select the best culture system for your needs * How to increase your chances of success
http://catalog2.corning.com/Lifesciences/media/pdf...
Added: Wed Feb 11 2009, Hits: 1199, Reviews: 0 Write review -
Guide For Identifying And Correcting Common Cell Growth Problems
(Corning)
This 14-page Corning Technical Guide reviews some of the common and not so common cell growth and attachment problems that are often very difficult to identify and eliminate.
http://catalog2.corning.com/Lifesciences/media/pdf...
Added: Wed Feb 11 2009, Hits: 1020, Reviews: 0 Write review -
Passaging Cells
(Dr. Volker Briese, Universitäts-Frauenklinik Rostock)
Basic cell culture procedures.
http://www-ufk.med.uni-rostock.de/lablinks/protoco...
Added: Mon Oct 28 2002, Hits: 889, Reviews: 0 Write review Cached -
Trypsinization of Adherent Cells
(Hannun/Obeid Lab, Duke Univ.)
Enzymatic method of passing cells.
http://www.duke.edu/web/ceramide/protocols/0005.ht...
Added: Wed Feb 11 2009, Hits: 1413, Reviews: 0 Write review Cached -
Trypsinizing and Subculturing Mammalian Cells
(Journal of Visualized Experiments)
As cells reach confluency, they must be subcultured or passaged. Failure to subculture confluent cells results in reduced mitotic index and eventually in cell death. The first step in subculturing is to detach cells from the surface of the primary culture vessel by trypsinization or mechanical means. The resultant cell suspension is then subdivided, or reseeded, into fresh cultures. Secondary cultures are checked for growth and fed periodically, and may be subsequently subcultured to produce tertiary cultures. The time between passaging of cells varies with the cell line and depends on the growth rate.
http://www.jove.com/index/details.stp?ID=755
Added: Wed Feb 11 2009, Hits: 1687, Reviews: 0 Write review Cached