Protocols
- Freezing and Thawing of MEFs (Shalini Jain and Hariom Yadav)
This protocol is very useful to the researchers working on primary mouse embryonic fibrobasts and gene cell lines
Added: Sun Aug 19 2007, Reviews: 0 Write review -
Freezing Cell and Thawing Cell
(Hannun/Obeid Lab)
http://www.musc.edu/bcmb/ceramide/protocols/0006.h...
Added: Tue May 14 2002, Hits: 2614, Reviews: 0 Write review Cached -
Freezing cells in liquid N2 for storage
(Antisense Research Group)
http://www.liv.ac.uk/~giles/methods/cell_freezing....
Added: Tue May 14 2002, Hits: 1642, Reviews: 0 Write review Cached -
General Guide for Cryogenically Storing Animal Cell Cultures
(Corning Life Science)
This 8-page Corning Technical Guide examines both the theoretical and practical aspects of cryogenic preservation and reviews key strategies for managing a cell repository
http://www.corning.com/lifesciences/technical_info...
Added: Thu Jul 15 2004, Hits: 3215, Reviews: 0 Write review Cached - Master Cell Bank (Contributed by Nanci Donacki)
Provides detailed protocol for establishing a master cell bank
Added: Tue May 14 2002, Reviews: 0 Write review -
Preparation of Lymphocyte Cell Pellet for Storage
(Donis-Keller lab)
Following propagation to 1 X 108 cells, lymphoblastoid cells are conveniently stored at -80 degrees C to preserve the high molecular weight DNA in the cells until the DNA is purified. This procedure describes the steps required to harvest and freeze the cells for long term storage.
http://humgen.wustl.edu/hdk_lab_manual/hcc/hcc5.ht...
Added: Tue May 14 2002, Hits: 939, Reviews: 0 Write review Cached -
Reactivating Frozen Cell Lines and Cell Growth for DNA Preparation
(Donis-Keller lab)
Cell lines are reactivated and grown to a count of 1 x 108 cells. The cells are pelleted and stored frozen at -80 degrees C prior to DNA extraction.
http://humgen.wustl.edu/hdk_lab_manual/hcc/hcc4.ht...
Added: Tue May 14 2002, Hits: 919, Reviews: 0 Write review Cached -
Reviving cells frozen in liquid N2
(Antisense Research Group)
http://www.liv.ac.uk/~giles/methods/cell_thawing.h...
Added: Tue May 14 2002, Hits: 973, Reviews: 0 Write review Cached -
Routine Splitting and freezing of cells
(Botwell Lab)
http://grimwade.biochem.unimelb.edu.au/bowtell/cel...
Added: Tue May 14 2002, Hits: 797, Reviews: 0 Write review Cached -
Thawing & Freezing Protocols for Insect Cells (BV)
(eBioscience)
A general protocol for insect cells.
http://www.ebioscience.com/ebioscience/appls/BVC.h...
Added: Mon Jan 02 2006, Hits: 332, Reviews: 0 Write review Cached