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• ES Stem Cell Freezing and Thawing

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Protocols

• Cryogenic Preservation and Storage of Animal Cells (Corning)
  This 5-page protocol provides a detailed procedure for cryogenically storing a variety of animal cells.
  http://catalog2.corning.com/Lifesciences/media/pdf...
  Added: Wed Feb 11 2009, Hits: 8535, Reviews: 0  Write review  
• CryoStor Cryopreservation Protocol (JoVE)
  This video protocol demonstrates the procedures for freezing, storage, and thawing of cells, as well as a viability assessment of the cells post-thaw.
  http://www.jove.com/details.php?id=2206
  Added: Mon May 16 2011, Hits: 450, Reviews: 0  Write review  
• General Guide For Cryogenically Storing Animal Cell Cultures (Corning)
  This 10-page Corning Technical Guide examines both the theoretical and practical aspects of cryogenic preservation and reviews key strategies for managing a cell repository. Special attention will be given to: - Understanding and controlling the freezing process - Selecting cryogenic storage systems - Record keeping - Quality control procedures to minimize culture loss
  http://catalog2.corning.com/Lifesciences/media/pdf...
  Added: Wed Feb 11 2009, Hits: 4229, Reviews: 0  Write review  
• Cell Storage Protocol (Hancock Laboratory Methods, Department of Microbiology and Immunology,University of British Columbia)
  Step by step method for storage of cell lines
  http://www.cmdr.ubc.ca/bobh/showmethod.php?methodi...
  Added: Sat Aug 31 2002, Hits: 5845, Reviews: 0  Write review   Cached
• Cell Thawing & Cell Freezing Protocol (Immunology Applications Core Facility, University of Chicago)
  Step by step method for freezing and thawing cells.
  http://iacf.bsd.uchicago.edu/MabHome/Cellthawfreez...
  Added: Mon Apr 19 2004, Hits: 933, Reviews: 0  Write review   Cached
• Freezer and Cell Line Database (contributed by Nanci Donacki)
  Very useful Microsoft Access database program (486kb) for managing your cell lines stored in freezer. It's easy to use and has a very nice screen. It has features like Add, Edit, Search and more. (To download it, for Windows explorer, click the file name; for Navigator, right click to save the link, for Mac, click and press on the file name until menu pops up and select to download.)
  http://www.protocol-online.org/protocols/cell_line...
  Added: Tue May 14 2002, Hits: 1973, Reviews: 0  Write review  
• Freezing and Thawing Cells (Gene Delivery Group, University of Birmingham)
  Simple protocol for freezing and thawing cells.
  http://web.bham.ac.uk/can4psd4/brum/freeze.html
  Added: Mon Sep 09 2002, Hits: 1353, Reviews: 0  Write review   Cached
• Freezing and Thawing cells (Sefton Lab, Salk Institute)
  http://pingu.salk.edu/~sefton/Hyper_protocols/free...
  Added: Tue May 14 2002, Hits: 3677, Reviews: 0  Write review   Cached
• Freezing and Thawing Cultured Cells (Allan Bradley's Lab, Baylor College of Medicine, Houston, Texas)
  http://www.imgen.bcm.tmc.edu/molgen/labs/bradley/f...
  Added: Sun Jul 28 2002, Hits: 2989, Reviews: 0  Write review   Cached
• Freezing and Thawing of Mammalian Cell Lines (Kitto Lab, The University of Texas at Austin)
  For long term storage of myeloma cells, hybridoma cells, T cells, and other mammalian cell lines in liquid nitrogen, and restoring them in culture.
  http://kitto.cm.utexas.edu/research/Kittolabpage/P...
  Added: Tue Oct 08 2002, Hits: 2970, Reviews: 0  Write review   Cached
• Freezing Cell and Thawing Cell (Hannun/Obeid Lab)
  http://www.musc.edu/bcmb/ceramide/protocols/0006.h...
  Added: Tue May 14 2002, Hits: 2411, Reviews: 0  Write review   Cached
• Freezing cells in liquid N2 for storage (Antisense Research Group)
  http://www.liv.ac.uk/~giles/methods/cell_freezing....
  Added: Tue May 14 2002, Hits: 1786, Reviews: 0  Write review  
• Master Cell Bank (Contributed by Nanci Donacki)
  Provides detailed protocol for establishing a master cell bank
  Added: Tue May 14 2002, Reviews: 0  Write review  
• Preparation of Lymphocyte Cell Pellet for Storage (Donis-Keller lab)
  Following propagation to 1 X 108 cells, lymphoblastoid cells are conveniently stored at -80 degrees C to preserve the high molecular weight DNA in the cells until the DNA is purified. This procedure describes the steps required to harvest and freeze the cells for long term storage.
  http://hg.wustl.edu/hdk_lab_manual/hcc/hcc5.html
  Added: Tue May 14 2002, Hits: 454, Reviews: 0  Write review   Cached
• Reactivating Frozen Cell Lines and Cell Growth for DNA Preparation (Donis-Keller lab)
  Cell lines are reactivated and grown to a count of 1 x 108 cells. The cells are pelleted and stored frozen at -80 degrees C prior to DNA extraction.
  http://hg.wustl.edu/hdk_lab_manual/hcc/hcc4.html
  Added: Tue May 14 2002, Hits: 421, Reviews: 0  Write review   Cached
• Reviving cells frozen in liquid N2 (Antisense Research Group)
  http://www.liv.ac.uk/~giles/methods/cell_thawing.h...
  Added: Tue May 14 2002, Hits: 1130, Reviews: 0  Write review  
• Routine Splitting and freezing of cells (Botwell Lab)
  http://grimwade.biochem.unimelb.edu.au/bowtell/cel...
  Added: Tue May 14 2002, Hits: 410, Reviews: 0  Write review   Cached
• Thawing Cells (Dr. Volker Briese, Universitäts-Frauenklinik Rostock)
  Schreiber's protocol for thawing cells.
  http://www-ufk.med.uni-rostock.de/lablinks/protoco...
  Added: Mon Oct 28 2002, Hits: 284, Reviews: 0  Write review   Cached
• Thawing Cells from Liquid Nitrogen (Gene Delivery Group, University of Birmingham)
  Detailed guide to cell thawing.
  http://web.bham.ac.uk/can4psd4/brum/liquid.html
  Added: Mon Sep 09 2002, Hits: 291, Reviews: 0  Write review   Cached
• Thawing Cells from Liquid Nitrogen (Hancock Laboratory Methods, Department of Microbiology and Immunology,University of British Columbia)
  http://www.cmdr.ubc.ca/bobh/showmethod.php?methodi...
  Added: Sat Aug 31 2002, Hits: 1070, Reviews: 0  Write review   Cached
• Working Cell Bank (Contributed by Nanci Donacki)
  Provides detailed protocol for establishing a working cell bank
  Added: Tue May 14 2002, Reviews: 0  Write review