Protocols

• Cell Cytotoxicity Assay (Dr. Volker Briese, Universitäts-Frauenklinik Rostock)
  To measure NK cell killing, suitable target cells are labeled with 51Cr, washed and incubated together with the killer cells (and treatments). Large amounts of label is released into the supernatant upon disruption of membrane integrity by the killing process and can be measured in a gamma-counter.
  http://www-ufk.med.uni-rostock.de/lablinks/protoco...
  Added: Mon Oct 28 2002, Hits: 700, Reviews: 0  Write review   Cached
• 3H Thymidine-Incorporation Assay for Rat-1a cells (iProtocol)
  http://iprotocol.mit.edu/protocol/128.htm
  Added: Mon Dec 09 2002, Hits: 254, Reviews: 0  Write review   Cached
• 3H-Thymidine Uptake by Cultured Cells (Dr. William H. Heidcamp, Biology Department, Gustavus Adolphus College)
  http://homepages.gac.edu/~cellab/chpts/chpt11/ex11...
  Added: Tue May 14 2002, Hits: 502, Reviews: 0  Write review   Cached
• 3H-Thymidine Uptake by Cultured Cells (iProtocol)
  A radioactive method for measuring cultured cell growth with 3H-thymidine.
  http://iprotocol.mit.edu/protocol/56.htm
  Added: Mon Dec 09 2002, Hits: 325, Reviews: 0  Write review   Cached
• 96-Well Cell Proliferation Assay (Promega)
  Colorimetric method for determining the number of viable cells in proliferation or chemosensitivity assays.
  http://www.promega.com/tbs/tb169/tb169.html#iv
  Added: Tue May 14 2002, Hits: 944, Reviews: 0  Write review   Cached
• Cell Proliferation Assay (Dr. Volker Briese, Universitäts-Frauenklinik Rostock)
  Cell proliferation assay by cell counting.
  http://www-ufk.med.uni-rostock.de/lablinks/protoco...
  Added: Mon Oct 28 2002, Hits: 885, Reviews: 0  Write review   Cached
• Cell Viability Assay (http://www.hallym.ac.kr/~gene)
  Dye exclusion method
  http://www.hallym.ac.kr/~gene/MoleGene/protocol/ki...
  Added: Tue May 14 2002, Hits: 1033, Reviews: 0  Write review   Cached
• Crystal Violet Assay (Dr. Volker Briese, Universitäts-Frauenklinik Rostock)
  This is a simple assay useful for obtaining quantitative information about the relative density of cells adhering to multi-well cluster dishes. The dye in this assay, crystal violet, stains DNA. Upon solubilization, the amount of dye taken up by the monolayer can be quantitated in a spectrophotometer or plate reader.
  http://www-ufk.med.uni-rostock.de/lablinks/protoco...
  Added: Mon Oct 28 2002, Hits: 621, Reviews: 0  Write review   Cached
• Cytotoxicity Assays Protocol (Hedrick Lab, UCSD Cancer Center)
  Cell-mediated cytotoxicity is determined by using a standard microcytotoxicity assay. Briefly, target cells were pelleted and resuspended in 100 mCi of Na51Cr...
  http://hedricklab.ucsd.edu/Protocol/cyto.html
  Added: Tue Dec 10 2002, Hits: 325, Reviews: 0  Write review   Cached
• Detection of BrdU Incorportation in DNA Synthesizing Cells (BD PharMingen)
  A standard protocol for detecting DNA in cells.
  http://www.bdbiosciences.com/pharmingen/protocols/...
  Added: Tue May 14 2002, Hits: 408, Reviews: 0  Write review   Cached
• Determination of IC50 (The Science Advisory Board)
  In vitro whole cell assay by [H3] Hypoxanthine uptake assay.
  http://www.scienceboard.net/resources/protocols.as...
  Added: Wed Apr 21 2004, Hits: 95, Reviews: 0  Write review   Cached
• Methods for Cell Death Staining (Steven Finkbeiner, Departments of Neurology and Physiology, UCSF)
  Includes trypan blue staining, propidium iodide staining of living cells and fixed cells, Hoescht staining.
  http://gweb1.ucsf.edu/labs/finkbeiner/Protocol/pro...
  Added: Tue Oct 01 2002, Hits: 1387, Reviews: 0  Write review   Cached
• MTT Anslysis (Gene Delivery Group, University of Birmingham)
  This procedure is for cells in 96 well plates.
  http://web.bham.ac.uk/can4psd4/brum/mtt.html
  Added: Mon Sep 09 2002, Hits: 915, Reviews: 2 Read reviews  Write review   Cached
• Proliferation Assay-[3H] Thymidine incorporation (Hannun/Obeid Lab)
  http://www.musc.edu/bcmb/ceramide/protocols/0017.h...
  Added: Tue May 14 2002, Hits: 493, Reviews: 0  Write review   Cached
• Soft Agar Assay (Stewart Lab, Washington University at St. Louis)
  
  http://www.stewartlab.wustl.edu/soft_agar_assay.ht...
  Added: Thu May 27 2004, Hits: 64, Reviews: 0  Write review   Cached
• Soft Agar Assay for Colony Formation (BioProtocol)
  http://www.bio.com/protocolstools/protocol.jhtml?i...
  Added: Thu May 27 2004, Hits: 53, Reviews: 0  Write review   Cached
• Soft Agar Assay For Colony Formation (Botwell Lab)
  http://grimwade.biochem.unimelb.edu.au/bowtell/cel...
  Added: Tue May 14 2002, Hits: 637, Reviews: 0  Write review   Cached
• Timing of Cycles (Dr. William H. Heidcamp, Biology Department, Gustavus Adolphus College)
  Determine Cell cycle by exposing cell to 3H-Thymidine, and subsequently fixing and autoradiography.
  http://homepages.gac.edu/~cellab/chpts/chpt11/ex11...
  Added: Tue May 14 2002, Hits: 227, Reviews: 0  Write review   Cached

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