Protocols
- Analysis of murine bone marrow: specimen choice, sampling and processing (Giorgio Cattoretti, MD, Columbia University )
Histologic analysis of murine BM is a necessary complement to flow cytometric or in vitro analysis. Techniques to do this are well established in human hematopathology. A long tradition in experimental hematology focuses on femur and tibia as the source of BM. However, the thickness of the cortical bone and the often preferred use of the largest bone, the femur, for cell culture or flow leaved to the pathologist a hard specimen with little marrow. Nice protocol with photos.
http://icg.cpmc.columbia.edu/cattoretti/Protocol/M...
Added: Sat Jul 27 2002, Hits: 854, Reviews: 0 Write review Cached -
Dissociation of spleen and hemopoietic tissue
(Giorgio Cattoretti, MD, Columbia University )
Dissociation of mouse cell
http://icg.cpmc.columbia.edu/cattoretti/Protocol/M...
Added: Sat Jul 27 2002, Hits: 647, Reviews: 0 Write review Cached -
Harvesting peritoneal cells
(Giorgio Cattoretti, MD, Columbia University )
Surgical procedure for collecting mouse peritoneal cells
http://icg.cpmc.columbia.edu/cattoretti/Protocol/M...
Added: Sat Jul 27 2002, Hits: 1304, Reviews: 0 Write review Cached -
Osteoblast Harvest from Mice
(SurgeryResearch.com)
Extract osteoblast cell from mice.
http://www.surgeryresearch.com/Protocols/OsteoMice...
Added: Wed Sep 25 2002, Hits: 341, Reviews: 0 Write review Cached -
Processing mouse tissue
(Giorgio Cattoretti, MD, Columbia University )
- Routine dissection
- Formalin fixation
- Storage of smears and slides
- Tissue embedding in paraffin
http://icg.cpmc.columbia.edu/cattoretti/Protocol/M...
Added: Sat Jul 27 2002, Hits: 1548, Reviews: 0 Write review Cached