Summary: In vivo imaging is a novel noninvasive technique for imaging biological processes in live animals and can provide insight into living organisms and helps to understand metabolic processes and disease related changes in the body. There are two commonly used in vivo imaging methods including bioluminescence imaging (BLI) and biofluorescence imaging (BFI), which enable monitoring of gene expression or disease progression in living organisms.
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Protocols
- In Vivo Luciferin Imaging Procedure
(Caliper)
Mice are injected by an intraperitoneal route with a Luciferin solution (15 mg/mL or 30 mg/kg, in PBS, dose of 150 mg/kg) that is allowed to distribute in awake animals for about 5-15 minutes. The mice are placed into a clear Plexiglas anesthesia box (2.5-3.5% isofluorane) that allows unimpeded visual monitoring of the animals; e.g. one can easily determine if the animals are breathing...
http://www.caliperls.com/assets/017/7560.pdf
Added: Wed Feb 04 2009, Hits: 2685, Reviews: 0 Write review -
Bioluminescence Imaging of Mice
(JoVE)
protocol for bioluminescent optical imaging of HO-1-luciferase transgenic mice provides a rapid in vivo assay of the upregulation of the heme oxygenase-1 (HO-1) gene expression in response to the Gua Sha procedure. HO-1 has long been known to provide cytoprotection against oxidative stress.
http://www.jove.com/index/details.stp?id=1385
Added: Sat Mar 20 2010, Hits: 1475, Reviews: 0 Write review Cached -
In vivo Bioluminescent Imaging of Mammary Tumors Using IVIS Spectrum
(JoVE)
Tumor growth can be monitored for several days by bioluminescence before the tumor size becomes palpable or measurable by traditional physical means. This rapid monitoring can provide insight into early events in tumor development or lead to shorter experimental procedures. Quantitative imaging of tumor growth using IVIS provides precise quantitation and accelerates the experimental process to generate results.
http://www.jove.com/index/Details.stp?ID=1210
Added: Sat Mar 20 2010, Hits: 1187, Reviews: 0 Write review Cached -
Noninvasive In Vivo Small Animal MRI and MRS: Basic Experimental Procedures
(JoVE)
Introduces basic procedures of mouse MRI/MRS experiments
http://www.jove.com/index/details.stp?id=1592
Added: Sat Mar 20 2010, Hits: 879, Reviews: 0 Write review Cached -
Preparation of Luciferin for In Vitro and In Vivo Bioluminescent Assays
(Caliper)
Luciferin is a substrate for luciferase and is injected into animals before in vivo imaging
http://www.caliperls.com/assets/017/7561.pdf
Added: Wed Feb 04 2009, Hits: 1632, Reviews: 0 Write review -
Xenogen Imager
(Fero Lab, Fred Hutchinson Cancer Research Center)
Describes methods for performing in vivo animal imaging including anesthesia and acquiring images.
http://labs.fhcrc.org/fero/Protocols/Xenogen.html
Added: Sun Feb 08 2009, Hits: 1203, Reviews: 0 Write review Cached