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Protocols
- Optimized Fibrin Gel Bead Assay for the Study of Angiogenesis
(Journal of Visualized Experiments)
In vitro angiogenesis assay that utilizes human umbilical vein EC and fibroblasts. This model recapitulates all of the key early stages of angiogenesis and, importantly, the vessels display patent intercellular lumens surrounded by polarized EC. EC are coated onto cytodex microcarriers and embedded into a fibrin gel. Fibroblasts are layered on top of the gel where they provide necessary soluble factors that promote EC sprouting from the surface of the beads. After several days, numerous vessels are present that can easily be observed under phase-contrast and time-lapse microscopy. This video demonstrates the key steps in setting up these cultures.
http://www.jove.com/index/details.stp?ID=186
Added: Sat Feb 07 2009, Hits: 1799, Reviews: 0 Write review Cached -
Chick Chorioallantoic Membrane (CAM) Assay
(Arispe Lab, UCLA)
http://www.mcdb.ucla.edu/Research/Arispe/Protocols...
Added: Thu Feb 19 2009, Hits: 1765, Reviews: 0 Write review -
Chorioallantoic Membrane (CAM) Assay
(Dario Neri's labs, Institute of Pharmaceutical Sciences ETHZ)
Method for CAM preparation
http://www.pharma.ethz.ch/institute_groups/biomacr...
Added: Sat Feb 07 2009, Hits: 1342, Reviews: 0 Write review Cached - Protocol for Aortic Ring Assay (Protocol Online)
A method of aortic ring assay to analyze micro-vessel outgrowth for angiogenesis analysis.
Added: Mon Feb 02 2009, Reviews: 0 Write review -
Vascular Permeability Assay
(Arispe Lab, UCLA)
http://www.mcdb.ucla.edu/Research/Arispe/Protocols...
Added: Thu Feb 19 2009, Hits: 2391, Reviews: 0 Write review