Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!

Video Options   
Photo

How to perform colony PCR



Rate Video   - - - - -

Couple points.  You must use a buffer that contains a detergent such as Taq buffer.  Most pfu buffers do not have a detergent and therefor will not efficiently break open the bacterial cells.  Also, be aware that false positives are a potential downfall of pcr screening.  When you add the ligation mixture to the competent cells during transformation, there is free insert DNA present.  That DNA gets spread onto the LB plate and can be picked up by your tip when picking a colony.  Always do a second diagnostic test once you have purified the plasmid.  


Videos System v3.0.1 © 2014 DevFuse
Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.