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Dear all,
I am new to enzymes. Got a topic to work with tryptophanase. However, the mutant tryptophanase is very unstable and it looses its activity within few hours in the crude extract. Hence, there is problem to purify it in its active form. I would like to know how can I characterize the enzyme in crude extract itself. Any suggestions to improve the stability of the enzyme is highly appreciated.
Thanks in advance,
Bindu S.
