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cardosopedro

Member Since 02 Jun 2009
Offline Last Active May 14 2013 04:52 AM

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Active Posts 123
Profile Views 6,992
Member Title Veteran
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My research interests
Programmed cell death

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Topics I've Started

Identifying new Ca2+-binding proteins

10 May 2013 - 12:20 AM

Hello.

I work with a model organism and based on my results I have some candidates proteins I believe are new Ca2+-binding molecules. How can I prove this? I don't have the recombinant protein (and I don't intend to produce them) but I have KO mutant cells for these genes/proteins. How could I prove, directly or indirectly, that these are in fact new Ca2+-related proteins?

So far I did growth analysis on culture medium with different concentrations of Ca2+ and these mutant cells behave differently from the wild type. I also transformed these cells with a genetic reporter that allows intracellular Ca2+ measurement.

Please help.

Thanks a lot!!!
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NADPH monitoring

22 April 2013 - 05:17 AM

Hello.

I would like to measure the levels of NADPH in my samples throughout time. I know there are some methods, but since I have no experience on this, I was wondering if someone could suggest me a good method. I work with a filamentous fungus (in case this is important to decide which is the best method). If possible I would like to do the assay on 96-well plates.

Thank you for the suggestions.
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